The Validity of the Salivary Cortisol Analysis Method Using the Cube Reader in Japanese University Students
The Validity of the Salivary Cortisol Analysis Method Using the Cube Reader in Japanese University Students
复制标题
使用 Cube Reader 进行唾液皮质醇分析方法在日本大学生中的有效性
DOI：
10.1111/jpr.12402
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发表时间：
2022
影响因子：
0.8
通讯作者：
Aoki Yoshiko
中科院分区：
文献类型：
--
作者：
Mitsuishi Hisashi;Okamura Hisayoshi;Moriguchi Yusuke;Aoki Yoshiko
唾液皮质醇的评估被认为是测量压力的一种非侵入性方法。为评价SOMA Cube Reader法测定唾液皮质醇的有效性，对18名健康日本大学生(包括非运动员)的唾液皮质醇测定结果与长期使用的酶联免疫吸附测定法(ELISA)进行了比较。分别于觉醒后即刻、觉醒后30 分钟、11：00、14：00、17：00测定唾液皮质醇水平。使用方差分析比较两种方法获得的测量结果。没有表现出显著的主效应或交互作用。在所有五个测试点的ELISA和Cube Reader测量结果、皮质醇唤醒反应、每日输出量和每日皮质醇斜率之间都注意到了显著的正相关(rS=5.50-0.75)。此外，通过Bland-Altman曲线图对Cube Reader和ELISA结果之间的一致性进行了评估，显示出良好的一致性，没有偏见。使用Cube Reader方法获得的结果与使用ELISA法获得的值相关联，证实了使用Cube Reader测量包括非运动员在内的健康日本大学生的每日唾液皮质醇水平的潜力。
The assessment of salivary cortisol is considered a noninvasive method for measuring stress. The validity of the SOMA Cube Reader method to measure salivary cortisol was evaluated by comparing the results with those of the long‐used enzyme‐linked immunosorbent assay method (ELISA) in 18 healthy Japanese university students (including non‐athletes). We measured diurnal salivary cortisol levels (immediately post‐awakening, 30 min post‐awakening, and at 11:00, 14:00, and 17:00). Measurements obtained by both methods were compared using analyses of variance. No significant main effects or interactions were demonstrated. Significant positive correlations were noted between the ELISA and Cube Reader measurements across all five testing points, the cortisol awakening response, the daily output, and the diurnal cortisol slope (rs = .50–.75). Moreover, the limits of agreement between the Cube Reader and the ELISA results were assessed via a Bland–Altman graph, showing good agreement with no bias. The results obtained with the Cube Reader method correlated with values obtained using the ELISA method, confirming the potential to use the Cube Reader for measuring diurnal salivary cortisol levels in healthy Japanese university students, including non‐athletes.