EGFR promotes the proliferation of quail follicular granulosa cells through the MAPK/extracellular signal-regulated kinase (ERK) signaling pathway
EGFR promotes the proliferation of quail follicular granulosa cells through the MAPK/extracellular signal-regulated kinase (ERK) signaling pathway
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EGFR通过MAPK/细胞外信号调节激酶（ERK）信号通路促进鹌鹑滤泡颗粒细胞增殖
DOI：
10.1080/15384101.2019.1656952
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发表时间：
2019-08
期刊：
影响因子：
4.3
通讯作者：
Du Jinping
中科院分区：
文献类型：
--
作者：
Wu Yan;Xiao Hongwei;Pi Jinsong;Zhang Hao;Pan Ailuan;Pu Yuejin;Liang Zhenhua;Shen Jie;Du Jinping
摘要：卵泡在卵泡发生过程中发育成排卵前卵泡，大多数黄色小卵泡会闭锁并被重新吸收。本研究根据鸭卵巢的RNA-seq结果，选择表皮生长因子受体（EGFR）作为卵泡发育的候选基因，并探讨其作用。结果表明EGFR-P8是鹌鹑EGFR核心启动子。它在 EGFR 核心启动子内有一个 E2F4 结合位点。 E2F4 过表达显着增加鹌鹑颗粒细胞 (GC) 中 EGFR 的表达。然而，当用 Corynoxeine（一种丝裂原激活蛋白激酶/细胞外调节蛋白激酶 (MAPK/ERK) 信号通路抑制剂）处理 GC 时，这种作用就消失了。此外，荧光素酶报告基因检测和染色质免疫沉淀实验表明，E2F4上调EGFR表达，从而增加E2和P4的产生。此外，EGFR 调节 GC 增殖并影响卵泡发育。综上所述，我们的研究结果表明，受 E2F4 调节的 EGFR 增强了 MAPK/ERK 通路成分的表达和卵泡发育。这些结果为加深对MAPK/ERK通路的理解和对鹌鹑卵泡发育的新认识提供了重要基础。
ABSTRACT Follicles develop into preovulatory follicles during folliculogenesis and the majority of small yellow follicles become atretic and gets reabsorbed. In this study, based the RNA-seq results of duck ovary, epidermal growth factor receptor (EGFR) was selected as a candidate gene in follicular development and the role was explored. The results demonstrated that EGFR-P8 was the quail EGFR core promoter. It had an E2F4 binding site within EGFR core promoter. E2F4 overexpression significantly increased EGFR expression in quail granulosa cells (GCs). However, the effect was abolished when the GCs were treated with corynoxeine, an inhibitor of the mitogen-activated protein kinase/extracellular regulated protein kinase (MAPK/ERK) signaling pathway. Moreover, luciferase reporter assay and chromatin immunoprecipitation experiments showed that E2F4 upregulated the expression of EGFR expression, which increased E2 and P4 production. In addition, EGFR regulated GCs proliferation and affected follicular development. Taken together, our findings suggested that EGFR, which was regulated by E2F4, enhanced the expression of MAPK/ERK pathway components and follicular development. These results provided an important basis for an improved understanding of the MAPK/ERK pathway and new insight into the development of quail follicles.
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DOI：
10.1126/science.1171396
发表时间：
2009-05-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Fan HY;Liu Z;Shimada M;Sterneck E;Johnson PF;Hedrick SM;Richards JS
通讯作者：
Richards JS
长非编码RNA GAS5通过抑制EZH2转录促进膀胱癌细胞凋亡。
影响因子：
9
作者：
Wang M;Guo C;Wang L;Luo G;Huang C;Li Y;Liu D;Zeng F;Jiang G;Xiao X
通讯作者：
Xiao X
影响因子：
4.6
作者：
Wei DW;Ma XY;Zhang S;Hong JY;Gui LS;Mei CG;Guo HF;Wang L;Ning Y;Zan LS
通讯作者：
Zan LS
影响因子：
--
作者：
Shimada, Masayuki;Hernandez-Gonzalez, Inmaculada;Richards, JoAnne S.
通讯作者：
Richards, JoAnne S.
影响因子：
6.4
作者：
J. Soriano;H. Uyttendaele;J. Kitajewski;R. Montesano
通讯作者：
J. Soriano;H. Uyttendaele;J. Kitajewski;R. Montesano