Rose MADS-box genes 'MASAKO C1 and D1' homologous to class C floral identity genes
Rose MADS-box genes 'MASAKO C1 and D1' homologous to class C floral identity genes
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DOI：
10.1016/s0168-9452(99)00206-x
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发表时间：
2000-02-21
期刊：
影响因子：
5.2
通讯作者：
Matsumoto, S
中科院分区：
文献类型：
--
作者：
Kitahara, K;Matsumoto, S
我们从野生玫瑰（Rosa rugosa Thunb，ex Murray）中分离到了AGAMOUS样cDNA克隆MASAKO C1-C6（C4-C6为3'或5'端部分cDNA克隆）和MASAKO D1。我们发现MASAKO C1是AGAMOUS的同源物，基于序列相似性、内含子位置的保守性以及生殖器官内雄蕊和心皮特异性表达。MASAKO C1-C6似乎是通过选择性剪接产生的，鉴于它与拟南芥中的ag-4变体2之间的相似性，讨论了不同mRNA之一“MASAKO C6”的可能功能。MASAKO D1也被认为是AGAMOUS的同源物，这是基于它们的序列的相似性，以及与GAG 2（人参中的AGAMOUS同源物）的组织特异性表达模式。两种类型的AGAMOUS同源基因可能存在于野生玫瑰中，正如在烟草、矮牵牛、黄瓜和玉米中观察到的那样。(C)2000由Elsevier Science爱尔兰有限公司出版。保留所有权利。
We isolated AGAMOUS-like cDNA clones called MASAKO C1-C6 (C4-C6 are 3' or 5' partial cDNA clones) and MASAKO D1 from wild rose (Rosa rugosa Thunb, ex Murray). We found that MASAKO C1 was a homologue of AGAMOUS on the basis of sequence similarity, conservation of intron positions, and stamen- and carpel-specific expression within reproductive organs. MASAKO C1-C6 seem to have arisen via alternative splicing, and a possible function of one of the different mRNAs 'MASAKO C6' is discussed in view of similarities between it and the ag-4 variant 2 within Alabidopsis thaliana. MASAKO D1 was also thought to be a homologue of AGAMOUS based on similarity of their sequences, and on the tissue specific expression pattern with GAG2, which is an AGAMOUS homologue in ginseng. Two types of AGAMOUS homologue genes are possibly present in wild rose, as has been observed in tobacco, petunia, cucumber, and maize. (C) 2000 Published by Elsevier Science Ireland Ltd. All rights reserved.