Enzyme assays with boronic acid appended bipyridinium salts.
Enzyme assays with boronic acid appended bipyridinium salts.
复制标题
使用硼酸附加联吡啶鎓盐进行酶测定。
DOI：
10.1016/j.aca.2009.07.032
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发表时间：
2009
影响因子：
6.2
通讯作者：
B. Singaram
中科院分区：
文献类型：
--
作者：
Boaz Vilozny;A. Schiller;R. Wessling;B. Singaram
In-vitro fluorescent enzyme assays have been developed for sucrose phosphorylase (SPO) and phosphoglucomutase (PGM). These assays make use of a selective carbohydrate sensing system that detects the unlabeled enzymatic products fructose and glucose-6-phosphate. The system comprises 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt as the reporter unit and boronic acid appended viologens as selective receptors with working ranges from 70μM to 1.0mM for fructose (SPO) and 190μM to 2.0mM for glucose-6-phosphate (PGM). The change in fluorescence can be converted into product concentration, allowing initial reaction velocities and Michaelis–Menten kinetics to be calculated. The assays are also carried out in multiwell plate formats, making them suitable for high-throughput screening of enzyme inhibitors. Rapid PGM inhibition screening is demonstrated with EDTA and LiCl. The PGM assay can also be used for enzyme quantification with a detection limit of 50ngmL−1.
影响因子：
3.9
作者：
Naught, LE;Tipton, PA
通讯作者：
Tipton, PA
影响因子：
2.9
作者：
Naught, LE;Regni, C;Tipton, PA
通讯作者：
Tipton, PA