Turnover rates of carbon and nitrogen stable isotopes in juvenile marbled flounder Pleuronectes yokohamae estimated by diet switch
Turnover rates of carbon and nitrogen stable isotopes in juvenile marbled flounder Pleuronectes yokohamae estimated by diet switch
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通过饮食转换估算横滨侧耳鲆幼鱼碳氮稳定同位素的周转率
DOI：
10.1007/s10228-015-0488-1
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发表时间：
2016
影响因子：
1.2
通讯作者：
Masakazu HORI
中科院分区：
文献类型：
--
作者：
Hideki Hamaoka;Jun SHOJI;Masakazu HORI
许多鱼种通常在不同生境之间迁移，因为它们对资源的需求发生了个体发生的变化;因此，澄清它们整个生命周期的生境之间的联系，对于管理和养护它们的种群十分重要。虽然碳（δ 13 C）和氮（δ 15 N）的稳定同位素比率被广泛用于阐明鱼类的迁徙，但信息仍然很少，包括大理石比目鱼（Pleuronectes yokohamae）在内的每种鱼类在迁徙期间转换为不同的饮食后如何改变其身体组织中的同位素组成。摘要本研究利用水族箱中的食物转换实验，探讨大理石鲽幼鱼阶段的同位素周转率。此外，我们在统计学上比较了批量样品（即，全身（不包括头部和内脏）到部分肌肉组织样本，以估计周转率的差异，作为饮食转换的指标。本文用指数模型描述了大理石鲽幼鱼δ 13 C和δ 15 N同位素速率的时间变化。肌肉组织和散装样品之间δ 13 C和δ 15 N变化的指数模型之间没有显著差异，肌肉和散装样品的δ 13 C和δ 15 N半衰期值相似（14.0-18.5天）。这些结果表明，散装样品是一个有用的替代肌肉组织的幼鱼的同位素分析和稳定同位素分析和估计的同位素周转率在组织中的大理石比目鱼可以用来澄清其在沿海地区的迁移路线。
Many fish species generally migrate among habitats because of ontogenetic changes in their resource requirements; therefore, clarifying linkages among habitats throughout their life cycle is important for the management and conservation of their populations. Although carbon (δ13C) and nitrogen (δ15N) stable isotope ratios are widely used for elucidating fish migrations, information is still scarce how each fish species, including marbled flounder (Pleuronectes yokohamae), change the isotopic composition in their body tissues after they switch to a different diet during migrations. This study clarified isotopic turnover rates of marbled flounder during the juvenile stage using a diet-switch experiment in an aquarium. Furthermore, we statistically compared bulk samples (i.e., whole body without head and viscera) to partial muscle tissue samples to estimate the difference in the turnover rate as an index for diet switches. An exponential model was used to describe the temporal changes in δ13C and δ15N isotopic rates of juvenile marbled flounder. No significant difference was noted between the exponential models for changes in both δ13C and δ15N between muscle tissue and bulk samples, and the half-life values were similar for muscle and bulk samples for both δ13C and δ15N (14.0–18.5 days). These results suggest that bulk samples are a useful substitute for muscle tissues in isotopic analysis of juvenile fish and that stable isotope analysis and the estimated isotopic turnover rates in tissues of marbled flounder can be used to clarify their migration route in coastal areas.