A microchip for quantitative analysis of CNS axon growth under localized biomolecular treatments.
A microchip for quantitative analysis of CNS axon growth under localized biomolecular treatments.
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DOI：
10.1016/j.jneumeth.2013.09.018
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发表时间：
2014-01-15
影响因子：
3
通讯作者：
Han, Arum
中科院分区：
文献类型：
--
作者：
Park, Jaewon;Kim, Sunja;Park, Su Inn;Choe, Yoonsuck;Li, Jianrong;Han, Arum
关键词：
神经元的生长能力是轴突再生的重要因素。为了更好地了解微环境如何影响轴突生长，迫切需要能够对细胞微环境进行空间控制并轻松量化轴突生长的方法。在这里，我们提出了一种微芯片，能够物理引导轴突的生长方向，同时提供与神经元胞体/树突的物理和流体隔离，从而实现局部生物分子治疗和线性轴突生长。即使在分离后，微芯片也允许轴突在轴突室内沿直线生长；因此，显着促进轴突长度量化过程。我们进一步开发了一种自动量化轴突生长的图像处理算法。研究了局部细胞外基质成分和脑源性神经营养因子治疗对轴突生长的影响。结果表明，生物分子可能对轴突生长产生显着不同的影响，具体取决于它们作用的部位。例如，虽然硫酸软骨素蛋白多糖在添加到轴突时会导致轴突回缩，但在应用于体细胞时会促进轴突生长。新开发的微芯片克服了传统轴突生长研究方法的局限性，这些方法缺乏对生物分子环境的局部控制，并且由于难以监测轴突生长，通常只能在短时间内以明显较低的细胞密度进行研究。这种微芯片可以作为研究促进轴突生长和再生的因素的有力工具。
Growth capability of neurons is an essential factor in axon regeneration. To better understand how microenvironments influence axon growth, methods that allow spatial control of cellular microenvironments and easy quantification of axon growth are critically needed. Here, we present a microchip capable of physically guiding the growth directions of axons while providing physical and fluidic isolation from neuronal somata/dendrites that enables localized biomolecular treatments and linear axon growth. The microchip allows axons to grow in straight lines inside the axon compartments even after the isolation; therefore, significantly facilitating the axon length quantification process. We further developed an image processing algorithm that automatically quantifies axon growth. The effect of localized extracellular matrix components and brain-derived neurotropic factor treatments on axon growth was investigated. Results show that biomolecules may have substantially different effects on axon growth depending on where they act. For example, while chondroitin sulfate proteoglycan causes axon retraction when added to the axons, it promotes axon growth when applied to the somata. The newly developed microchip overcomes limitations of conventional axon growth research methods that lack localized control of biomolecular environments and are often performed at a significantly lower cell density for only a short period of time due to difficulty in monitoring of axonal growth. This microchip may serve as a powerful tool for investigating factors that promote axon growth and regeneration.
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影响因子：
6.1
作者：
Park J;Koito H;Li J;Han A
通讯作者：
Han A
影响因子：
3.3
作者：
Robert, F.;Cloix, J-F.;Hevor, T.
通讯作者：
Hevor, T.
影响因子：
4.2
作者：
Serra, Margarida;Leite, Sofia B.;Alves, Paula M.
通讯作者：
Alves, Paula M.
影响因子：
5.3
作者：
Ng, Benjamin K.;Chen, Lian;Chan, Jonah R.
通讯作者：
Chan, Jonah R.
DOI：
10.1126/science.1161566
发表时间：
2008-11-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Park KK;Liu K;Hu Y;Smith PD;Wang C;Cai B;Xu B;Connolly L;Kramvis I;Sahin M;He Z
通讯作者：
He Z