Optimization of detection sensitivity in the analysis of inorganic cations by capillary ion electrophoresis using indirect photometric detection
Optimization of detection sensitivity in the analysis of inorganic cations by capillary ion electrophoresis using indirect photometric detection
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DOI：
10.1016/0021-9673(92)87147-z
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发表时间：
1992-09
影响因子：
4.1
通讯作者：
A. Weston;P. Brown;P. Jandík;A. Heckenberg;W. R. Jones
中科院分区：
文献类型：
--
作者：
A. Weston;P. Brown;P. Jandík;A. Heckenberg;W. R. Jones
毛细管离子电泳（沃茨的商品名为毛细管离子分析，或CIA）是毛细管区带电泳的细分，其中分离条件针对无机或低分子量离子的分析进行了优化。1991年首次报道了用毛细管离子电泳法分离碱金属、碱土金属和过渡金属[LC · GC，9（1991）634]，表明这些阳离子在10 min内分离，检测限为中ppb，分离效率超过100 000理论塔板。此后，通过改变硬件和电解质成分，检测灵敏度得到了提高。这些变化导致检测限降低了一个数量级以上，使痕量分析在许多应用中变得实用。并讨论了该方法良好的重现性和线性。
Capillary ion electrophoresis (Waters' trade name Capillary Ion Analysis, or CIA) is the subdivision of capillary zone electrophoresis in which the conditions for the separation are optimized for the analysis of inorganic or low-molecular-mass ions. The first reported separation of alkali, alkaline earth and transition metals by capillary ion electrophoresis, published in 1991 [LC · GC, 9 (1991) 634], showed the separation of these cations in under 10 min with mid-ppb detection limits and separation efficiencies of more than 100 000 theoretical plates. Detection sensitivity has since been improved by altering both hardware and electrolyte components. These alterations have resulted in a decrease in detection limits of more than an order of magnitude, making trace level analyses practical for many applications. The good reproducibility and linearity of this technique are also discussed.