Time course of motoneurone death after neonatal sciatic nerve crush in the rat.
Time course of motoneurone death after neonatal sciatic nerve crush in the rat.
复制标题
大鼠新生坐骨神经挤压后运动神经元死亡的时间过程。
DOI：
10.1159/000112120
复制
发表时间：
1994
影响因子：
2.9
通讯作者：
C. Davies
中科院分区：
文献类型：
--
作者：
M. Lowrie;D. Lavalette;C. Davies
坐骨神经运动神经元逆行标记与持久的荧光染料之前，单侧神经压榨在3日龄或成年大鼠。存活标记的运动神经元的数量在神经损伤后的间隔进行了比较，在对侧控制腹角和未手术的动物的数量。成年神经挤压后，标记的运动神经元的数量没有显着减少，但新生儿神经挤压后，计数减少到约35%。大部分细胞死亡发生在神经损伤后的前6天，主要来自运动柱的下半部分，但约三分之一的细胞死亡发生在6 - 12天，主要来自上半部分。这些结果表明，不太成熟的运动神经元往往死亡最早，在肌肉再神经支配。那些在上层的，更成熟的部分的车辆存活时间更长，但仍然可能在神经再生过程中死亡。至少有两种类型的胶质细胞被二次标记的这种方法，区分他们对神经损伤的反应。
Sciatic motoneurones were retrogradely labelled with long-lasting fluorescent dyes prior to unilateral nerve crush in either 3-day-old or adult rats. The number of surviving labelled motoneurones at intervals after nerve injury were compared to the number in the contralateral control ventral horn and in unoperated animals. Following adult nerve crush there was no significant reduction in the number of labelled motoneurones, but after neonatal nerve crush the count was reduced to about 35%. Most of the cell death occurred during the first 6 days after nerve injury, mainly from the lower half of the motor column, but about one third died between 6 and 12 days, mainly from the upper part. These results suggest that less mature motoneurones tend to die earliest, before the muscle is reinnervated. Those in the upper, more mature part of the motor pool survive longer but may still die during reinnervation. At least two types of glial cell were secondarily labelled by this method, distinguished by their response to nerve injury.