Protective effects of quercetin and vitamin C against oxidative stress-induced neurodegeneration
Protective effects of quercetin and vitamin C against oxidative stress-induced neurodegeneration
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DOI：
10.1021/jf049243r
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发表时间：
2004-12-15
影响因子：
6.1
通讯作者：
Lee, CY
中科院分区：
文献类型：
--
作者：
Heo, HJ;Lee, CY
几种神经退行性疾病的临床试验越来越多地以评估各种抗氧化剂的有效性为目标。人类的饮食中含有数千种植物化学物质，其中许多具有显著的生物活性。维生素C是一种天然的抗氧化剂，众所周知，它可以降低阿尔茨海默病等神经退行性疾病的风险。槲皮素是一些水果和蔬菜中的主要黄酮类化合物之一，具有比维生素C更强的抗氧化和抗癌活性。因此，我们研究了槲皮素对过氧化氢诱导的神经变性的保护作用。为了确定氧化应激对PC12细胞的保护作用，我们在过氧化氢处理PC12细胞前预先加入了槲皮素和维生素C，结果表明，在氧化应激作用下，PC12细胞的存活率明显高于维生素C，并且具有比维生素C更强的保护作用。我们观察到，槲皮素比维生素C更能减少氧化应激诱导的神经细胞膜损伤。这些结果表明，除了许多其他生物益处外，栎皮素还对神经元细胞免受氧化应激诱导的神经毒性(如阿尔茨海默病)的保护作用做出了重要贡献。
Clinical trials of several neurodegenerative diseases have increasingly targeted the evaluation of various antioxidants' effectiveness. The human diet contains several thousand phytochemicals, many of which have significant bioactivities. Vitamin C, a naturally occurring antioxidant, is known to reduce the risk of neurodegenerative disorders such as Alzheimer's disease. Quercetin, one of the major flavonoids in some fruits and vegetables, has much stronger antioxidative and anticarcinogenic activities than vitamin C. Therefore, we investigated the protective effects of quercetin on hydroxy peroxide-induced neurodegeneration. To determine the protective effects, PC12 cells were preincubated with quercetin and vitamin C before H2O2 treatment for 2 h. Results showed that cell viability was clearly improved with quercetin, and quercetin showed a higher protective effect than vitamin C. Because oxidative stress is known to increase neuronal cell membrane breakdown, we further investigated lactate dehydrogenase and trypan blue exclusion assays. We observed that quercetin decreased oxidative stress-induced neuronal cell membrane damage more than vitamin C. These results suggest that quercetin, in addition to many other biological benefits, contributes significantly to the protective effects of neuronal cells from oxidative stress-induced neurotoxicity, such as Alzheimer disease.