Reply: Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques
Reply: Mechanical Micronization of Lipoaspirates: Squeeze and Emulsification Techniques
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回复：脂肪抽吸物的机械微粉化：挤压和乳化技术
DOI：
10.1097/prs.0000000000003373
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发表时间：
2017
影响因子：
3.6
通讯作者：
Yoshimura K
中科院分区：
文献类型：
--
作者：
Mashiko T;Wu SH;Feng J;Kanayama K;Yoshimura K
背景：移植脂肪的凝结被认为是脂肪移植后获得更好结果的关键。作者研究了两种机械组织微粉程序的治疗潜力：挤压和乳化。方法：将人抽吸脂肪离心（离心脂肪），用自动切片机切碎（挤压脂肪）。或者，离心后的脂肪通过小孔连接器在两个注射器之间反复转移乳化，然后通过网状过滤分离成两部分：乳化脂肪的残余组织和乳化脂肪的滤液。检测了这四种产品的细胞成分。结果：组织学和电镜分析显示，挤压脂肪和乳化脂肪残余组织中含有破裂的脂肪细胞和断裂的毛细血管。与离心脂肪相比，压缩脂肪和残余脂肪产品的比重增加，每体积脂肪来源的干细胞/基质细胞和内皮细胞数量增加，表明乳化脂肪的压缩脂肪和残余组织都成功地进行了细胞/组织凝聚。尽管在挤压脂肪和残余脂肪中基质血管部分的细胞数量和活力都保持得很好，但基质血管部分培养实验表明，脂肪来源的基质细胞在乳化脂肪的残余组织中相对受损，而在挤压脂肪中则没有受损。相比之下，没有脂肪来源的基质细胞从乳化脂肪过滤液培养。结论：作者的研究结果表明，机械微化是一种简单的操作程序，可以通过选择性地去除脂肪细胞来浓缩组织，而不会损害脂肪来源的基质细胞和内皮细胞等关键成分。根据脂肪细胞去除的程度，该产品可能是有效组织体积化或治疗性再生/受精的有用治疗工具。
Background:Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The authors investigated the therapeutic potential of two mechanical tissue micronizing procedures: squeeze and emulsification.Methods:Human aspirated fat was centrifuged (centrifuged fat) and fragmented with an automated slicer (squeezed fat). Alternatively, centrifuged fat was emulsified by repeated transfer between two syringes through a small-hole connecter and then separated by mesh filtration into two portions: residual tissue of emulsified fat and filtrated fluid of emulsified fat. The four products were examined for cellular components.Results:Histologic and electron microscopic analyses revealed that squeezed fat and residual tissue of emulsified fat contained broken adipocytes and fragmented capillaries. Compared with centrifuged fat, the squeezed fat and residual fat products exhibited increased specific gravity and increased numbers of adipose-derived stem/stromal cells and endothelial cells per volume, suggesting successful cell/tissue condensation in both squeezed fat and residual tissue of emulsified fat. Although cell number and viability in the stromal vascular fraction were well maintained in both squeezed fat and residual fat, stromal vascular fraction culture assay showed that adipose-derived stromal cells were relatively damaged in residual tissue of emulsified fat but not in squeezed fat. By contrast, no adipose-derived stromal cells were cultured from filtrated fluid of emulsified fat.Conclusions:The authors’ results demonstrated that mechanical micronization is easily conducted as a minimal manipulation procedure, which can condense the tissue by selectively removing adipocytes without damaging key components, such as adipose-derived stromal cells and endothelial cells. Depending on the extent of adipocyte removal, the product may be a useful therapeutic tool for efficient tissue volumization or therapeutic revitalization/fertilization.