The bacterial microbiome in paired vaginal and vestibular samples from women with vulvar vestibulitis syndrome
The bacterial microbiome in paired vaginal and vestibular samples from women with vulvar vestibulitis syndrome
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DOI：
10.1111/2049-632x.12197
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发表时间：
2014-12-01
影响因子：
3.3
通讯作者：
Forney, Larry J.
中科院分区：
文献类型：
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作者：
Jayaram, Aswathi;Witkin, Steven S.;Forney, Larry J.
采用焦磷酸测序法比较了30例外阴前庭炎综合征（VVS）女性和15例健康对照者阴道和前庭细菌微生物群的组成。ELISA法检测阴道白细胞介素-1 β浓度。问卷调查获得临床和症状数据。在VVS女性的阴道样本中检测到18个属，在前庭样本中鉴定出23个属。VVS受试者在两个位点的属数和平均属数与对照组基本相似。然而，不同之处在于，VVS女性中链球菌和肠球菌的比例更高。此外，乳酸菌在VVS女性中更常见，而crispatus在对照组女性中更常见。在两组中，阴道中的优势细菌属与相应前庭样本中的优势细菌属密切平行，这使我们假设阴道分泌物是前庭上存在的细菌的重要来源。阴道IL-1 β水平相似，并根据优势细菌而变化。我们在这项初步研究中得出结论，除了在一些VVS女性中链球菌和L. iners的患病率增加外，阴道和前庭在有或没有VVS的女性中没有明显的主要差异。
Composition of the bacterial microbiome in the vagina and vestibule from 30 women with vulvar vestibulitis syndrome (VVS) and 15 healthy controls were compared by pyrosequencing 16S rRNA gene amplicons. Vaginal concentrations of interleukin (IL)-1 beta were determined by ELISA. Questionnaires elicited clinical and symptom data. Eighteen genera were detected in vaginal samples, and 23 genera were identified in vestibule samples, from women with VVS. The genera at both sites and the mean number of genera in subjects with VVS were largely similar to those in control subjects. However, differences were noted including higher proportions of Streptococcus and Enterococcus in women with VVS. Furthermore, Lactobacillus iners was more frequently identified in women with VVS while L. crispatus was more frequent in the control women. The dominant bacterial genera in the vagina closely paralleled the dominant genera present in the corresponding vestibular sample in both groups, leading us to postulate that vaginal secretions are an important source of bacteria present on the vestibule. Vaginal IL-1 beta levels were similar and varied depending on the dominant bacteria. We conclude in this pilot study that no major differences are apparent in the vagina and vestibule between women with or without VVS, except for an increased prevalence of Streptococcus and L. iners in some women with VVS.