THE SEQUENCE A-ALPHA-(148-160) IN FIBRIN, BUT NOT IN FIBRINOGEN, IS ACCESSIBLE TO MONOCLONAL-ANTIBODIES
THE SEQUENCE A-ALPHA-(148-160) IN FIBRIN, BUT NOT IN FIBRINOGEN, IS ACCESSIBLE TO MONOCLONAL-ANTIBODIES
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DOI：
10.1073/pnas.86.22.8951
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发表时间：
1989-11-01
影响因子：
11.1
通讯作者：
NIEUWENHUIZEN, W
中科院分区：
文献类型：
--
作者：
SCHIELEN, WJG;VOSKUILEN, M;NIEUWENHUIZEN, W
纤维蛋白原，而不是纤维蛋白原，加速组织型纤溶酶原激活剂催化的纤溶酶原激活。先前的工作表明，纤维蛋白的这种加速能力的基本信息存在于对应于A α的残基148-160的序列中。纤维蛋白原链[A α-（148-160）]。基于这些发现，我们的工作假设是A α-（148-160）包埋在纤维蛋白原中，当纤维蛋白原转化为纤维蛋白时，变得可接近蛋白质如纤溶酶原和/或组织型纤溶酶原激活物。为了检验这一假设，我们提出了一种抗合成A α-（148-160），发现该抗体与纤维蛋白反应而不与纤维蛋白原反应。这一发现表明A α.（148-160）中所述的蛋白质在纤维蛋白原转化为纤维蛋白时变得可接近，并且A α-（148-160）是纤维蛋白特异性新抗原决定簇。
Fibrin, but not fibrinogen, accelerates the activation of plasminogen catalyzed by tissue-type plasminogen activator. Previous work showed that essential information for this accelerating capacity of fibrin resides in the sequence corresponding to residues 148-160 of the A.alpha. chain of fibrinogen [A.alpha.-(148-160)]. Our working hypothesis, based on those findings, is that A.alpha.-(148-160) is buried in fibrinogen and becomes accessible to proteins such as plasminogen and/or tissue-type plasminogen activator when fibrinogen is converted to fibrin. To test this hypothesis we have raised a monoclonal antibody against synthetic A.alpha.-(148-160) and found that this antibody reacts with fibrin and not with fibrinogen. This finding shows that A.alpha.(148-160) becomes accessible when fibrinogen is converted to fibrin and that A.alpha.-(148-160) is a fibrin-specific neoantigenic determinant.