Synthetic oligonucleotides recreate Drosophila fushi tarazu zebra-stripe expression.
Synthetic oligonucleotides recreate Drosophila fushi tarazu zebra-stripe expression.
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合成寡核苷酸重现果蝇 fushi tarazu 斑马条纹表达。
DOI：
10.1101/gad.5.5.855
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发表时间：
1991
影响因子：
10.5
通讯作者：
Parker,CS
中科院分区：
文献类型：
--
作者：
Topol,J;Dearolf,CR;Prakash,K;Parker,CS
位于fushi tarazu（ftz）转录起始位点近端669 bp内的一系列复杂的激活子和阻遏子元件足以产生ftz基因特有的“斑马条纹”表达模式。P-元件介导的转化和ftz启动子/lacZ融合基因被用来表征，详细地说，这些转录控制元件。通过重建启动子与合成的寡核苷酸含有顺式调节条纹表达，我们表明，这些监管网站可以作为独立的单位，直接在果蝇胚胎的位置特异性转录。特别是，我们证明了多个拷贝的正调控位点可以介导表达的奇数和偶数parasegments在整个大部分的胚芽带和负调控位点可以转化为离散条纹的基因表达的连续模式。提出的重建启动子系统提供了一个有效的手段，研究在早期胚胎空间限制转录的分子机制。
A complex array of activator and repressor elements located within 669 bp proximal to the fushi tarazu (ftz) transcriptional start site is sufficient to generate the "zebra-stripe" expression pattern characteristic of the ftz gene. P-element-mediated transformation and ftz promoter/lacZ fusion genes were used to characterize, in detail, several of these transcriptional control elements. By reconstructing promoters with synthetic oligonucleotides containing cis-regulators of stripe expression, we show that these regulatory sites can function as independent units to direct position-specific transcription in the Drosophila embryo. In particular, we demonstrate that multiple copies of a positive regulatory site can mediate expression in both the odd- and even-numbered parasegments throughout most of the germ band and that negative regulatory sites can transform a continuous pattern of gene expression into discrete stripes. The reconstructed promoter system presented provides an effective means of studying molecular mechanisms governing spatially restricted transcription in the early embryo.