miR-149 promotes the myocardial differentiation of mouse bone marrow stem cells by targeting Dab2
miR-149 promotes the myocardial differentiation of mouse bone marrow stem cells by targeting Dab2
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DOI：
10.3892/mmr.2018.8903
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发表时间：
2018-06-01
影响因子：
3.4
通讯作者：
Chen, Tao
中科院分区：
文献类型：
--
作者：
Lu, Mingjun;Xu, Lingling;Chen, Tao
为了研究微小RNA（miR）-149在体外小鼠骨髓间充质干细胞（MSC）心脏分化中的作用，用过表达miR-149的慢病毒感染MSC，并确定其对心脏分化的影响。定量聚合酶链反应结果表明，miR-149促进MSCs中心脏特异性标志物的表达。蛋白质印迹和荧光素酶活性测定表明，失效同源物 2 (Dab2) 是 miR-149 的直接靶标。 Dab2 异位表达和 Wnt/-catenin 信号通路抑制能够逆转 miR-149 诱导的心脏特异性标志物表达增加。总之，miR-149能够靶向Dab2并在体外促进小鼠MSC的心脏分化，这依赖于Wnt/-catenin信号通路。
To investigate the role of microRNA (miR)-149 in the cardiac differentiation of mouse bone marrow mesenchymal stem cells (MSCs) in vitro, MSCs were infected with a lentivirus overexpressing miR-149 and the effect on cardiac differentiation was determined. The quantitative polymerase chain reaction results demonstrated that miR-149 promoted the expression of cardiac-specific markers in MSCs. Western blotting and a luciferase activity assay demonstrated that disabled homolog 2 (Dab2) was a direct target of miR-149. Dab2 ectopic expression and Wnt/-catenin signaling pathway inhibition was able to reverse the increased expression of cardiac-specific markers induced by miR-149. In conclusion, miR-149 was able to target Dab2 and promote the cardiac differentiation of mouse MSCs in vitro, which depended upon the Wnt/-catenin signaling pathway.