Mechanisms of inactivation of hepatitis A virus in water by chlorine dioxide
Mechanisms of inactivation of hepatitis A virus in water by chlorine dioxide
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DOI：
10.1016/j.watres.2003.12.021
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发表时间：
2004-03-01
期刊：
影响因子：
12.8
通讯作者：
Chao, FH
中科院分区：
文献类型：
--
作者：
Li, JW;Xin, ZT;Chao, FH
本研究为阐明二氧化氯灭活甲型肝炎病毒（HAV）的机制，采用细胞培养、酶联免疫吸附试验（ELISA）和长重叠RT-PCR检测消毒前后HAV的感染性、抗原性和全基因组。结果显示，接触每升 7.5 毫克二氧化氯 10 分钟后，感染性完全失活； 5'非翻译区（5'NTR）（第1至671位序列）敏感性最高，其灭活时间与二氧化氯灭活HAV感染性的时间一样长；接触每升 7.5 毫克二氧化氯 10 分钟后，抗原性完全破坏。提示二氧化氯对HAV的灭活机制是由于5'NTR的丢失和/或抗原性的破坏，这与氯的灭活机制不同(Appl Environ Microbiol 68:4951)。 (C) 2004 Elsevier Ltd. 保留所有权利。
In this study, to elucidate the mechanisms of inactivation of hepatitis A virus (HAV) by chlorine dioxide, cell culture, enzyme-linked immunosorbent assay (ELISA), and long-overlapping RT-PCR were used to detect the infectivity, antigenicity, and entire genome of HAV before and after disinfection. The results revealed the complete inactivation of infectivity after a 10-min exposure to 7.5 mg of chlorine dioxide per liter; and the highest level of sensitivity in the 5' non-translated regions (5'NTR) (the sequence from bp 1 to 671), inactivation of which took as much time as the inactivation of infectivity of HAV by chlorine dioxide; the complete destruction of antigenicity after a 10-min exposure to 7.5 mg of chlorine dioxide per liter. It is suggested that the inactivation mechanism of HAV by chlorine dioxide was due to the loss of the 5'NTR and/or destruction of the antigenicity, which is not similar to that of chlorine (Appl Environ Microbiol 68: 4951). (C) 2004 Elsevier Ltd. All rights reserved.