Traffic-related air pollution induces non-allergic eosinophilic airway inflammation and cough hypersensitivity in guinea-pigs
Traffic-related air pollution induces non-allergic eosinophilic airway inflammation and cough hypersensitivity in guinea-pigs
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与交通相关的空气污染引起豚鼠非过敏性嗜酸性粒细胞气道炎症和咳嗽过敏
DOI：
10.1111/cea.13308
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发表时间：
2019-03-01
影响因子：
6.1
通讯作者：
Lai, Kefang
中科院分区：
文献类型：
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作者：
Fang, Zhangfu;Huang, Chuqin;Lai, Kefang
背景嗜酸性粒细胞增多相关的慢性咳嗽如非哮喘性嗜酸性支气管炎和咳嗽变异性哮喘的发病机制和病理生理学尚不清楚。目的本研究旨在探讨交通相关空气污染（TRAP）在嗜酸性粒细胞炎症和咳嗽反应中的潜在作用。方法将非致敏豚鼠在城市交通隧道中暴露于TRAP或在过滤空气环境中饲养7或7天。 14天。分别使用柠檬酸和异硫氰酸烯丙酯（AITC）挑战来测量反射性咳嗽。使用录音和波形分析来确定自发咳嗽计数。使用支气管肺泡灌洗液（BALF）中的差异细胞和肺组织病理学评估气道炎症。为了进一步阐明气道炎症和咳嗽过敏之间的关系，暴露 14 天的一组动物接受了地塞米松治疗。结果与未暴露动物的 AITC 激发引起的反射性咳嗽计数（10 分钟内的平均值（95% 置信区间））（3.1（1.7-4.5））相比，在 7 天（12.0 （6.8-17.2），P
BackgroundThe pathogenesis and pathophysiology of eosinophilia-related chronic cough such as non-asthmatic eosinophilic bronchitis and cough variant asthma are still not clear.ObjectiveThis study is to examine the potential role of traffic-related air pollution (TRAP) in eosinophilic inflammation and cough responses.MethodsNon-sensitized guinea-pigs were exposed to TRAP in an urban traffic tunnel or kept in a filtered air environment for 7 or 14days. Reflexive cough was measured using citric acid and allyl isothiocyanate (AITC) challenges, respectively. Spontaneous cough counting was determined using audio recording and a waveform analysis. Airway inflammation was evaluated using differential cells in bronchoalveolar lavage fluid (BALF) and lung histopathology. To further elucidate the relationship between airway inflammation and cough hypersensitivity, a subgroup of those exposed for 14days received a dexamethasone treatment.ResultsCompared to reflexive cough count (mean (95% confidence interval) in 10minutes) provoked by the AITC challenge for the unexposed animals (3.1 (1.7-4.5)), those were increased significantly following both the 7-day (12.0 (6.8-17.2), P