Characterization of Chromosome Inheritance of the Intergeneric BC2 and BC3 Progeny between Saccharum spp. and Erianthus arundinaceus.
Characterization of Chromosome Inheritance of the Intergeneric BC2 and BC3 Progeny between Saccharum spp. and Erianthus arundinaceus.
复制标题
甘蔗属间 BC2 和 BC3 后代的染色体遗传特征。
DOI：
10.1371/journal.pone.0133722
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Huang Y;Wu J;Wang P;Lin Y;Fu C;Deng Z;Wang Q;Li Q;Chen R;Zhang M
斑茅具有生物量高、活力强、配给能力强、耐旱耐涝、抗病虫害等优良的甘蔗改良农艺性状。为了研究高代甘蔗基因组导入甘蔗的情况，以甘蔗属间产生的BC2和BC3代为材料，对高代甘蔗BC2和BC3进行了研究。和高羊茅的基因组原位杂交(GISH)技术进行研究。结果表明，BC2和BC3代是n+n染色体传递的结果。此外，在一些甘蔗后代中，染色体易位发生在高粱染色体的末端片段。和高羊茅E.arundinaceus。值得注意的是，易位的染色体可以稳定地传递给他们的后代。本研究对甘蔗属间后代BC2和BC3的染色体遗传特性进行了研究。和高羊茅E.arundinaceus。本研究可为斑茅种质资源研究提供更多有用的分子细胞遗传学信息，为甘蔗育种工作者进一步了解斑茅种质资源，促进其在甘蔗商品化育种中的应用提供参考。
Erianthus arundinaceus (E. arundinaceus) has many desirable agronomic traits for sugarcane improvement, such as high biomass, vigor, rationing ability, tolerance to drought, and water logging, as well as resistance to pests and disease. To investigate the introgression of the E. arundinaceus genome into sugarcane in the higher generations, intergeneric BC2 and BC3 progeny generated between Saccharum spp. and E. arundinaceus were studied using the genomic in situ hybridization (GISH) technique. The results showed that the BC2 and BC3 generations resulted from n + n chromosome transmission. Furthermore, chromosome translocation occurred at terminal fragments from the E. arundinaceus chromosome in some progeny of Saccharum spp. and E. arundinaceus. Notably, the translocated chromosomes could be stably transmitted to their progeny. This study illustrates the characterization of chromosome inheritance of the intergeneric BC2 and BC3 progeny between Saccharum spp. and E. arundinaceus. This work could provide more useful molecular cytogenetic information for the germplasm resources of E. arundinaceus, and may promote further understanding of the germplasm resources of E. arundinaceus for sugarcane breeders to accelerate its progress in sugarcane commercial breeding.