Expression of retinoid receptors during human monocyte differentiation in vitro
Expression of retinoid receptors during human monocyte differentiation in vitro
复制标题
DOI：
10.1006/bbrc.2000.2371
复制
发表时间：
2000-04-02
影响因子：
3.1
通讯作者：
Kreutz, M
中科院分区：
文献类型：
--
作者：
Fritsche, J;Stonehouse, TJ;Kreutz, M
1 α,25-二羟基维生素 D-3 (1,25(OH)(2)VD3) 和视黄酸 (RA) 调节单核细胞 (MO) 的激活及其向巨噬细胞 (MAC) 的分化。由于这些效应主要是由 1,25(OH)(2)VD3 和 RA 特定核受体的异二聚体或同二聚体介导，因此我们研究了 MO 分化为 MAC 或树突状细胞 (DC) 期间视黄酸受体 (RAR) α、β 和 γ 以及类视黄醇 X 受体 (RXR) a 的表达。在短期培养的 MO 中检测到除 RAR beta 之外的所有研究受体的 mRNA。在 MO 分化为 MAC 的过程中，RA 受体的 mRNA 表达下降。相比之下，沿着MO向DC的分化途径，只有RARγ的mRNA表达下降，而RARα和RXRα则持续高水平表达。尽管在 MO 衍生的 DC 中 RAR α 和 RXR α 在 mRNA 水平上强烈表达，但这些细胞中受体的蛋白表达较低。然而，MO 和 MO 衍生的 MAC 在蛋白质水平上显示出这些受体的强烈表达。这表明受体调节的转录后或翻译后机制正在这些细胞中发生，特别是在 DC 中。 MAC 和 DC 之间 RA 受体表达和蛋白水平的反向调节可能控制这些细胞对 1,25(OH)(2)VD3 和 RA 的反应性。 (C) 2000 年学术出版社。
1 alpha,25-Dihydroxyvitamin D-3 (1,25(OH)(2)VD3) and retinoic acid (RA) modulate the activation of monocytes (MO) and their differentiation into macrophages (MAC). As these effects are mostly mediated by heterodimers or homodimers of the specific nuclear receptors for 1,25(OH)(2)VD3 and RA, we investigated the expression of the retinoic acid receptors (RAR) alpha, beta, and gamma and the retinoid X-receptor (RXR) a in MO during differentiation into MAC or dendritic cells (DC). The mRNA of all investigated receptors except RAR beta was detected in short-term cultured MO. During differentiation of MO to MAC the mRNA expression of the RA receptors decreased. In contrast, along the differentiation pathway of MO to DC, only the mRNA expression of RAR gamma declined, whereas RAR alpha and RXR alpha were constantly expressed at a high level. Despite the strong expression of RAR alpha and RXR alpha at mRNA level in MO-derived DC, the protein expression of the receptors was low in these cells. However, MO and MO-derived MAC showed a strong expression of these receptors at protein level. This suggests that a posttranscriptional or posttranslational mechanism of receptor regulation is occurring in these cells, and in particular in the DC. The inverse regulation of RA receptor expression and protein levels between MAC and DC may control the responsiveness of these cells to 1,25(OH)(2)VD3 and RA. (C) 2000 Academic Press.