Gene expression profile of the regeneration epithelium during axolotl limb regeneration.

Gene expression profile of the regeneration epithelium during axolotl limb regeneration.
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DOI:
10.1002/dvdy.22669
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发表时间:
2011-07
影响因子:
2.5
通讯作者:
Crews, Craig M.
Crews, Craig M.
中科院分区:
生物学3区
文献类型:
--
作者:
Campbell, Leah J.;Suarez-Castillo, Edna C.;Ortiz-Zuazaga, Humberto;Knapp, Dunja;Tanaka, Elly M.;Crews, Craig M.

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有尾目两栖动物在成年脊椎动物中是独一无二的,因为它们具有再生缺失肢体的能力。肢体再生过程需要几个关键组织,包括具有再生能力的伤口表皮,称为再生上皮 (RE)。我们使用微阵列分析来分析墨西哥蝾螈中 RE 的基因表达。 125 个基因和表达序列标签 (EST) 的列表显示,RE 中的表达量比覆盖侧袖口伤口的伤口表皮中的表达量高 1.5 倍。 RE EST 和基因的子集进一步表征了再生过程中表达水平的变化。这项研究首次大规模鉴定了 RE 中的特定基因表达。
Urodele amphibians are unique amongst adult vertebrates in their ability to regenerate missing limbs. The process of limb regeneration requires several key tissues including a regeneration-competent wound epidermis called the regeneration epithelium (RE). We used microarray analysis to profile gene expression of the RE in the axolotl, a Mexican salamander. A list of 125 genes and expressed sequence tags (ESTs) showed a ≥1.5 fold expression in the RE than in a wound epidermis covering a lateral cuff wound. A subset of the RE ESTs and genes were further characterized for expression level changes over the time-course of regeneration. This study provides the first large scale identification of specific gene expression in the RE.
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