Peptide detection in single cells using a dot immunoblot assay
Peptide detection in single cells using a dot immunoblot assay
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DOI：
10.1016/s0196-9781(97)00006-5
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发表时间：
1997-01-01
期刊：
影响因子：
3
通讯作者：
Tublitz, NJ
中科院分区：
文献类型：
--
作者：
Loi, PK;McGraw, HF;Tublitz, NJ
本文介绍了一种用于检测和定量测定单细胞中小分子肽的高灵敏度斑点免疫印迹法（DIA）。该DIA方案简单、快速，并且不产生放射性废物。它的毫微微摩尔灵敏度是以前描述的DIA的100倍。该DIA方法足够灵敏，以允许以比其他肽检测程序更快和更容易的方式从单个细胞获得可靠的肽测量。该方法也可用于几个其他目的，包括评估抗体特异性和肽定量。(C)1997年爱思唯尔科学公司
A highly sensitive dot immunoblot assay (DIA) for the detection and quantitative measurement of small peptides in single cells is presented. This DIA protocol is simple, rapid, and produces no radioactive waste. Its femtomole sensitivity is 100 fold greater than previously described DIAs. This DIA method is sufficiently sensitive to allow reliable peptide measurements to be obtained from a single cell in a manner than is faster and easier than other peptide detection procedures. This method can also be used for several other purposes, including assessing antibody specificity and peptide quantification. (C) 1997 Elsevier Science Inc.