Healing of artificial fenestration defects by seeding of fibroblast-like cells derived from regenerated periodontal ligament in a dog:: A preliminary study
Healing of artificial fenestration defects by seeding of fibroblast-like cells derived from regenerated periodontal ligament in a dog:: A preliminary study
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DOI：
10.1089/10763270360728099
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发表时间：
2003-12-01
影响因子：
--
通讯作者：
Oygür, T
中科院分区：
文献类型：
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作者：
Dogan, A;Özdemir, A;Oygür, T
本研究的目的是评估成纤维细胞样细胞的播种对犬人工开窗缺陷牙周愈合的促进作用。成纤维细胞样细胞是通过将手术取出的再生牙周韧带组织置于特氟龙膜下进行培养培养的。在上颌尖牙和第一磨牙上以5 ~ 5mm的间距手术诱导开窗缺损。将2 × 10(5)个传代的自体凝血细胞置于两个缺损的根表面；剩下的两个缺陷作为控制。术后第42天组织形态学评估愈合情况。4个样本中有3个样本的主要牙周愈合模式为结缔组织适应，包括1个对照组，牙骨质形成率为9-12%；一个对照标本显示100%骨质形成。细胞播种组的新骨形成率（84%）高于对照组（39%）。在细胞播种组，一个标本显示出完全的骨再生（100%）；然而，观察到位于新形成的骨和根表面之间的结缔组织适应牙本质表面，牙骨质形成有限。从牙周韧带中提取细胞用于牙周再生可能是有希望的，但需要进一步的研究。
The purpose of this study was to assess the seeding of fibroblast-like cells to promote periodontal healing in artificial fenestration defects in a dog. Fibroblast-like cells were cultured by incubating regenerated periodontal ligament tissue, that had been surgically taken, underneath a Teflon membrane. Fenestration defects were surgically induced on the maxillary canine and first molar teeth at a spacing of 5 to 5 mm. Passage 4 cells (2 X 10(5) cells) in autologous blood coagulum were placed on root surfaces in two defects; the remaining two defects were used as controls. Healing was evaluated histomorphometrically on postoperative day 42. The main periodontal healing pattern consisted of connective tissue adaptation in three of the four specimens including one control, with cementum formation at 9-12%; one control specimen that exhibited 100% cementum formation. New bone formation was greater in the cell-seeding group (84%) compared with control (39%). In the cell-seeding group, one specimen exhibited total regeneration of bone (100%); however, the connective tissue located between newly formed bone and the root surface was observed to adapt to the dentin surface, with limited cementum formation. Seeding of cells from periodontal ligament may be promising to promote periodontal regeneration, but needs to be investigated in further studies.