Activation of phospholipase D by the fucose-sulfate glycoconjugate that induces an acrosome reaction in spermatozoa.
Activation of phospholipase D by the fucose-sulfate glycoconjugate that induces an acrosome reaction in spermatozoa.
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DOI：
10.1016/s0021-9258(18)60547-2
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发表时间：
1989-06
期刊：
影响因子：
--
通讯作者：
S. Domino;S. Bocckino;D. Garbers
中科院分区：
文献类型：
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作者：
S. Domino;S. Bocckino;D. Garbers
我们首次报道了海胆精子中磷脂酶D的活性可以通过一种已知能诱导顶体反应的果冻成分来调节。在精子中诱导顶体反应的卵磷脂的焦糖-硫酸糖缀合物（FSG）引起Ca2+依赖性的1,2-二酰基甘油和磷脂酸的增加。在精子中加入FSG后，二酰基甘油浓度增加了2倍，磷脂酸浓度增加了10倍。FSG也引起胆碱浓度升高，但没有引起磷酸胆碱浓度升高。在顶体反应中，二酰基甘油的磷酸化和甘油的新合成都不是合成磷脂酸的重要途径。当精子与FSG在乙醇存在下孵育时，产生磷脂酰乙醇。当细胞外培养基中的乙醇浓度从0.1增加到2.5%时，磷脂酰乙醇的数量增加，而磷脂酸的浓度下降，表明磷脂酶d催化了竞争性转磷脂酰化反应。此外，当精子中的磷脂酰胆碱池用[3H]1- o-烷基-2-裂解甘油-3-磷酸胆碱进行放射性标记时，随后添加的FSG导致[3H]磷脂酸的积累增加了4倍。fsg诱导的[3H]磷脂酸升高与发生顶体反应的细胞百分比呈正相关。
We report for the first time that phospholipase D activity in sea urchin spermatozoa can be regulated by a component of egg jelly known to induce an acrosome reaction. The fucose-sulfate glycoconjugate (FSG) of egg jelly that induces an acrosome reaction in spermatozoa caused Ca2+-dependent increases in 1,2-diacylglycerol and phosphatidic acid. Diacylglycerol concentrations were increased 2-fold, and phosphatidic acid concentrations were elevated up to 10-fold 2 min after the addition of FSG to spermatozoa. FSG also caused increases in choline, but not in choline phosphate concentrations. Neither phosphorylation of diacylglycerol norde novosynthesis from glycerol were significant routes of synthesis of phosphatidic acid during the acrosome reaction. When spermatozoa were incubated with FSG in the presence of ethanol, phosphatidylethanol was produced. As ethanol concentrations in the extracellular medium were increased from 0.1 to 2.5%, the amount of phosphatidylethanol increased, whereas phosphatidic acid concentrations decreased, suggesting a competitive transphosphatidylation reaction catalyzed by phospholipase D. Furthermore, when a phosphatidylcholine pool in spermatozoa was radiolabeled using [3H]1-O-alkyl-2-lyso-glycerol-3-phosphorylcholine, the subsequent addition of FSG caused a 4-fold accumulation of [3H]phosphatidic acid. FSG-induced elevations in [3H]phosphatidic acid were positively correlated with the percent of cells that had undergone an acrosome reaction.