Enhanced Angiogenesis by Transplantation of Mesenchymal Stem Cell Sheet Created by a Novel Magnetic Tissue Engineering Method
Enhanced Angiogenesis by Transplantation of Mesenchymal Stem Cell Sheet Created by a Novel Magnetic Tissue Engineering Method
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DOI：
10.1161/atvbaha.111.231100
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发表时间：
2011-10-01
影响因子：
8.7
通讯作者：
Murohara, Toyoaki
中科院分区：
文献类型：
--
作者：
Ishii, Masakazu;Shibata, Rei;Murohara, Toyoaki
细胞移植治疗性血管生成是治疗严重缺血性疾病的一种新策略。然而，一些患者对这种常规的基于注射的血管生成细胞疗法的反应较差。在这里，我们调查了一种治疗潜力的间充质干细胞（MSC）片由一种新的磁铁矿组织工程技术修复angiogenesis.Methods和Results-human MSCs温育与磁性纳米粒子含有脂质体进行培养，磁铁被放置在反面。磁化后的骨髓间充质干细胞在磁力作用下形成多层细胞片。裸小鼠进行单侧后肢缺血并分成3组。对照组在缺血组织内注射生理盐水。在MSC注射组中，小鼠通过常规针注射而不使用片状配方接受磁化MSC作为对照细胞组。在MSC-片层组中，MSC片层在皮肤闭合之前覆盖在缺血组织上。血流恢复和血管生成的程度分别通过激光多普勒血流仪和组织学毛细血管密度进行评估。与对照组和MSC注射组相比，MSC片组在缺血组织中具有更大的血管生成。MSC片的血管生成和组织保护作用归因于血管内皮生长因子表达的增加和缺血组织中细胞凋亡的减少。在培养的骨髓间充质干细胞，磁标记本身抑制细胞凋亡通过过氧化氢酶样抗氧化mechanism.Conclusion-MSC片创建的新的磁性纳米粒子为基础的组织工程技术将代表一种新的治疗性血管生成和组织再生的方式。（Arterioscler Thromb Vasc Biol.2011;31：2210-2215.）
Objective-Therapeutic angiogenesis with cell transplantation represents a novel strategy for severe ischemic diseases. However, some patients have poor response to such conventional injection-based angiogenic cell therapy. Here, we investigated a therapeutic potential of mesenchymal stem cell (MSC) sheet created by a novel magnetite tissue engineering technology for reparative angiogenesis.Methods and Results-Human MSCs incubated with magnetic nanoparticle-containing liposomes were cultured, and a magnet was placed on the reverse side. Magnetized MSCs formed multilayered cell sheets according to magnetic force. Nude mice were subjected to unilateral hind limb ischemia and separated into 3 groups. For the control group, saline was injected into ischemic tissue. In the MSC-injected group, mice received magnetized MSCs by conventional needle injections without sheet formula as a control cell group. In the MSC-sheet group, MSC sheet was layered onto the ischemic tissues before skin closure. Blood flow recovery and the extent of angiogenesis were assessed by a laser Doppler blood flowmetry and histological capillary density, respectively. The MSC-sheet group had a greater angiogenesis in ischemic tissues compared to the control and MSC-injected groups. The angiogenic and tissue-preserving effects of MSC sheets were attributable to an increased expression of vascular endothelial growth factor and reduced apoptosis in ischemic tissues. In cultured MSCs, magnetic labeling itself inhibited apoptosis via a catalase-like antioxidative mechanism.Conclusion-MSC sheet created by the novel magnetic nanoparticle-based tissue engineering technology would represent a new modality for therapeutic angiogenesis and tissue regeneration. (Arterioscler Thromb Vasc Biol. 2011;31:2210-2215.)