Pre- and postmenopausal women have different core urinary microbiota.
Pre- and postmenopausal women have different core urinary microbiota.
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DOI：
10.1038/s41598-021-81790-8
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发表时间：
2021-01-26
影响因子：
4.6
通讯作者：
Sørensen S
中科院分区：
文献类型：
--
作者：
Ammitzbøll N;Bau BPJ;Bundgaard-Nielsen C;Villadsen AB;Jensen AM;Leutscher PDC;Glavind K;Hagstrøm S;Arenholt LTS;Sørensen S
最近的研究表明，女性尿液微生物群的改变与膀胱疾病的发展有关。然而，正常的微生物群组成和健康妇女的变化描述甚少。此外，更年期期间激素变化对微生物群的影响尚不清楚。本研究的目的是调查无尿路症状的健康绝经前和绝经后妇女的尿微生物群。采用16S rRNA基因测序技术绘制尿标本微生物群组成图。最初总共包括41名绝经前和42名绝经后妇女。排除首次PCR扩增浓度低于阴性对照的样本，纳入34名绝经前妇女和20名绝经后妇女进行数据分析。与绝经前妇女相比，绝经后妇女的尿液显示出明显更高的α多样性。乳杆菌是两组中最丰富的细菌，但乳杆菌的相对丰度在绝经前妇女中占77.8%，在绝经后妇女中占42.0%。综上所述，绝经前妇女的尿液主要以乳酸杆菌为主，而绝经后妇女的尿液微生物群更为多样化，加德纳菌属和普雷沃菌属的丰度更高。这种差异的临床和病理生理意义仍有待阐明。
Recent studies suggest that alterations in the female urinary microbiota is associated to development of bladder disease. However, the normal microbiota composition and variation in healthy women are poorly described. Moreover, the effects of hormonal changes on microbiota during menopause is not well understood. The aim of our study was to investigate the urinary microbiota in healthy pre- and postmenopausal women without urinary tract symptoms. Microbiota composition in catheterized urine samples was mapped using 16S rRNA gene sequencing. In total, 41 premenopausal and 42 postmenopausal women were initially included. Samples with first PCR amplification concentration below level of the negative control were excluded, resulting in 34 premenopausal and 20 postmenopausal women included in data analysis. Urine from postmenopausal women showed significantly higher alpha diversity compared to premenopausal women. Lactobacillus was the most abundant bacteria in both groups, however the relative abundance of Lactobacillus accounted for 77.8% in premenopausal versus 42.0% in postmenopausal women. In conclusion, urine from premenopausal mostly presented with Lactobacillus dominated urotypes, whereas urine from postmenopausal women presented a more diverse urinary microbiota with higher abundance of the genera Gardnerella and Prevotella. The clinical and pathophysiological implications of this difference remain to be elucidated.