Primate epididymis-specific proteins: characterization of ESC42, a novel protein containing a trefoil-like motif in monkey and human.
Primate epididymis-specific proteins: characterization of ESC42, a novel protein containing a trefoil-like motif in monkey and human.
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DOI：
10.1210/endo.142.10.8422
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发表时间：
2001-10
期刊：
影响因子：
4.8
通讯作者：
Qiang Liu;K. G. Hamil;P. Sivashanmugam;G. Grossman;Rama Soundararajan;A. Rao;R. Richardson;Yonglian Zhang;M. O'Rand;Peter Petrusz;Frank S. French;S. H. Hall
中科院分区：
文献类型：
--
作者：
Qiang Liu;K. G. Hamil;P. Sivashanmugam;G. Grossman;Rama Soundararajan;A. Rao;R. Richardson;Yonglian Zhang;M. O'Rand;Peter Petrusz;Frank S. French;S. H. Hall
附睾分泌蛋白通过与精子相互作用促进精子成熟和受精。在这里，我们通过分离新的附睾特异性表达序列的cdna克隆来研究精子成熟的分子基础。从猕猴附睾消减文库中分离并测序了36个新的cDNA。这些克隆编码具有一系列基序的蛋白质，这些基序包括蛋白质修饰酶、蛋白酶抑制剂、疏水配体结合和运输蛋白、细胞外基质相互作用蛋白和转录调节因子。获得了11个克隆的全长编码序列，代表了丰度水平的范围。每种基因的表达都是区域性的，并受雄激素的调节。最丰富的ESC42含有一个富含半胱氨酸的区域，类似于三叶草家族的运动创伤修复蛋白的标志性结合域。猴子和人类的蛋白质几乎90%相同。免疫组织化学染色显示，该蛋白在头部上皮细胞中含量最丰富，也存在于管腔中并与精子结合。ESC42基因位于染色体20q11上，包含两个外显子，编码两个几乎相同的预测信号肽和第三个外显子，编码蛋白质的其余部分。
Epididymal secreted proteins promote sperm maturation and fertilizing capacity by interacting with sperm during passage through the epididymis. Here we investigate the molecular basis of sperm maturation by isolating cDNA clones for novel epididymis-specific expressed sequences. Thirty-six novel cDNAs were isolated and sequenced from a subtracted Macaca mulatta epididymis library. The clones encode proteins with a range of motifs characteristic of protein-modifying enzymes, protease inhibitors, hydrophobic ligand-binding and transport proteins, extracellular matrix-interacting proteins, and transcription regulatory factors. The full length coding sequences were obtained for 11 clones representing a range of abundance levels. Expression of each is regionally localized and androgen regulated. The most abundant, ESC42, contains a cysteine-rich region similar to the signature binding domain of the trefoil family of motogenic wound repair proteins. The monkey and human proteins are nearly 90% identical. Immunohistochemical staining revealed that the protein is most abundant in the epithelium of the caput and is also present in the lumen and bound to sperm. The ESC42 gene, located on chromosome 20q11, contains two exons encoding two nearly identical predicted signal peptides and a third exon encoding the rest of the protein.