Induction of mucosal and systemic immune responses by intranasal immunization using recombinant cholera toxin B subunit as an adjuvant.
Induction of mucosal and systemic immune responses by intranasal immunization using recombinant cholera toxin B subunit as an adjuvant.
复制标题
使用重组霍乱毒素 B 亚基作为佐剂，通过鼻内免疫诱导粘膜和全身免疫反应。
DOI：
10.1016/s0264-410x(97)00168-0
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发表时间：
1998
期刊：
影响因子：
5.5
通讯作者：
Russell,MW
中科院分区：
文献类型：
--
作者：
Wu,HY;Russell,MW
鼻内（i.n.）用含有微量霍乱毒素（CT）的与霍乱毒素B亚单位（CT B）混合的变形链球菌表面蛋白AgI II免疫诱导粘膜和全身部位的强免疫应答，但单独的纯CT B是否具有佐剂作用一直受到质疑。为了确定重组（r）CTB的佐剂作用，用与5 μg rCTB混合或缀合的10 μg AgI II免疫小鼠，并通过ELISA测定唾液、鼻洗液、肠洗液、阴道洗液和血清中的抗体应答。结果表明，AgI II无论是与rCTB混合还是与rCTB结合，都可以诱导粘膜伊加和全身IgG抗体达到比单独用AgI II类似免疫的小鼠更高的水平。某些反应，特别是血清IgG抗体，通过向免疫原中添加5 μg CT而增强，而用AgI II与CT污染的CTB混合免疫的总体小鼠倾向于产生对AgI II的最强粘膜伊加和血清IgG反应。然而，与有意或无意地与CT混合的CTB相比，用作佐剂的rCTB诱导的针对自身的抗体应答较低。这些结果表明，rCTB可以作为通过i.n.路线
Intranasal (i.n.) immunization with Streptococcus mutans surface protein AgI II mixed with cholera toxin B subunit (CTB) containing a trace amount of cholera toxin (CT) induces strong immune responses in mucosal and systemic sites, but whether pure CTB alone has an adjuvant effect has been questioned. To determine the adjuvant effect of recombinant (r) CTB, mice were immunized with 10 μg of AgI II either mixed with or conjugated to 5 μg of rCTB, and antibody responses in saliva, nasal wash, gut wash, vaginal wash, and serum were assayed by ELISA. The results showed that AgI II either mixed with or conjugated to rCTB could induce both mucosal IgA and systemic IgG antibodies to higher levels than in mice similarly immunized with AgI II alone. Some responses, especially serum IgG antibodies, were enhanced by adding 5 μg of CT to the immunogen, whereas overall mice immunized with AgI II mixed with CTB contaminated with CT tended to generate the strongest mucosal IgA and serum IgG responses to AgI II . However, rCTB used as an adjuvant induced lower antibody responses against itself than CTB intentionally or inadvertently mixed with CT. These results show that rCTB can serve as an adjuvant for protein immunogens administered by the i.n. route.