Effect of the surface morphology of silk fibroin scaffolds for bone regeneration
Effect of the surface morphology of silk fibroin scaffolds for bone regeneration
复制标题
DOI：
10.3233/bme-161595
复制
发表时间：
2016-01-01
影响因子：
1
通讯作者：
Nishiyama, Norihiro
中科院分区：
文献类型：
--
作者：
Bhawal, Ujjal K.;Uchida, Ryoichiro;Nishiyama, Norihiro
现有的支架不能充分满足骨再生的同时要求。挑战仍然是如何改善新形成的骨与周围组织的整合。本研究的目的是研究两种丝素蛋白支架，六氟异丙醇基丝素蛋白（HFIP-F）和水基丝素蛋白（A-F），对体内大临界尺寸骨缺损的骨诱导潜力的影响。β-磷酸三钙（β-TCP）用作阳性对照。植入兔膝关节缺损处1周和2周后，苏木精-伊红（HE）染色和Azan染色显示A-F支架和β-TCP比HFIP-F支架具有更强的骨诱导能力。A-F支架显示出含有骨样结节的新组织的突出区域。此外，在兔膝关节骨缺损内的天然组织和新组织之间观察到诱导的骨整合。免疫组织化学染色显示A-F支架植入物中Runx 2、BMP-2、BMP-7、Smad 1/5/9和Phospho-Smad的表达最高。多孔A-F支架的骨诱导可能受到植入物中局部微环境中存在的BMP信号传导量的影响。该研究为使用A-F丝素蛋白支架进行骨缺损再生开辟了一条新途径。
Existing scaffolds cannot adequately satisfy the simultaneous requirements for the regeneration of bone. The challenge remains of how to improve the integration of newly formed bone with the surrounding tissues. The purpose of this study was to investigate the effects of two silk fibroin scaffolds, a hexafluoro isopropanol-based silk fibroin (HFIP-F) and an aqueous-based silk fibroin (A-F), for their osteoinductive potentials in large critical size bone defects in vivo. beta-tricalcium phosphate (beta-TCP) was used as a positive control. After implantation into defects created in the knee joints of rabbits for 1 and 2 weeks, hematoxylin and eosin (H-E) and Azan staining revealed that the A-F scaffold as well as beta-TCP had stronger osteoinductive ability than the HFIP-F scaffold. The A-F scaffold exhibited prominent areas of neo-tissue containing bone-like nodules. Furthermore, induced osteointegration was observed between native and neo-tissue within the osteo defects in the knee joints of rabbits. Immunohistochemical staining showed the highest expression of Runx2, BMP-2, BMP-7, Smad1/5/9 and Phospho-Smad in the A-F scaffold implants. Osteoinduction of the porous A-F scaffold might be influenced by the amount of BMP signaling present in the local microenvironment in the implants. This study opens a new avenue to use A-F silk fibroin scaffolds for the regeneration of bone defects.