Confirmation of the pleiotropic control of leaflet shape and number of seeds per pod by the Ln gene in induced soybean mutants.
Confirmation of the pleiotropic control of leaflet shape and number of seeds per pod by the Ln gene in induced soybean mutants.
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DOI：
10.1270/jsbbs.16201
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发表时间：
2017-09
期刊：
影响因子：
2.4
通讯作者：
Ishimoto M
中科院分区：
文献类型：
--
作者：
Sayama T;Tanabata T;Saruta M;Yamada T;Anai T;Kaga A;Ishimoto M
大多数大豆品种具有宽的小叶；然而，已知Ln位点上的隐性等位基因会导致小叶从宽变窄。隐性等位基因ln也被认为可以增加每荚种子数（NSP），并具有提高产量的潜力。最近，一个控制Ln的基因Gm-JAG1 （Glyma20g25000）被证明可以在拟南芥突变体中补充叶片形状和叶片长度。然而，Gm-JAG1是否对大豆的这些性状负责尚不清楚。本研究利用2个独立的大豆Gm-jag1突变体和4个近等基因系（NILs），研究了大豆Ln基因对小叶形状和NSP的多效性影响。使用SmartGrain定制的叶片图像分析软件SmartLeaf评估叶片形状。Gm-jag1突变体的小叶都比野生型长、窄。有趣的是，图像分析结果表明，突变小叶的周长没有改变，尽管它们的小叶面积减少了。此外，一个窄小叶突变系的NSP显著高于野生（或Ln）基因型，表明大豆Ln基因多效性控制小叶形状和NSP。
Most soybean cultivars possess broad leaflets; however, a recessive allele on the Ln locus is known to cause the alteration of broad to narrow leaflets. The recessive allele ln has also been considered to increase the number of seeds per pod (NSP) and has the potential to improve yield. Recently, Gm-JAG1 (Glyma20g25000), a gene controlling Ln, has been shown to complement leaf shape and silique length in Arabidopsis mutants. However, whether Gm-JAG1 is responsible for those traits in soybean is not yet known. In this study, we investigated the pleiotropic effect of soybean Ln gene on leaflet shape and NSP by using two independent soybean Gm-jag1 mutants and four ln near isogenic lines (NILs). The leaflet shape was evaluated using a leaf image analysis software, SmartLeaf, which was customized from SmartGrain. The leaflets of both the Gm-jag1 mutants were longer and narrower than those of the wild-type plants. Interestingly, the image analysis results clarified that the perimeter of the mutant leaflets did not change, although their leaflet area decreased. Furthermore, one mutant line with narrow leaflets showed significantly higher NSP than that in the wild (or Ln) genotype, indicating that soybean Ln gene pleiotropically controls leaflet shape and NSP.
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DOI：
10.1266/jjg.9.208
发表时间：
1934-01-01
期刊：
JAP JOUR GENETICS
影响因子：
--
作者：
TAKAHASHI, NOBORU
通讯作者：
TAKAHASHI, NOBORU
影响因子：
0.7
作者：
Tsukaya, H
通讯作者：
Tsukaya, H
影响因子：
7.2
作者：
Yano, Ryoichi;Takagi, Kyoko;Ishimoto, Masao
通讯作者：
Ishimoto, Masao
影响因子：
7.4
作者：
Tanabata, Takanari;Shibaya, Taeko;Yano, Masahiro
通讯作者：
Yano, Masahiro
影响因子：
2.3
作者：
MANDL, FA;BUSS, GR
通讯作者：
BUSS, GR