Kanglaite sensitizes colorectal cancer cells to Taxol via NF-κΒ inhibition and connexin 43 upregulation.
Kanglaite sensitizes colorectal cancer cells to Taxol via NF-κΒ inhibition and connexin 43 upregulation.
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康莱特通过 NF-κ 抑制和连接蛋白 43 上调使结直肠癌细胞对紫杉醇敏感
DOI：
10.1038/s41598-017-01480-2
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发表时间：
2017-04-28
影响因子：
4.6
通讯作者：
Hou S
中科院分区：
文献类型：
--
作者：
Wang Y;Zhang C;Zhang S;Zhao Z;Wang J;Song J;Wang Y;Liu J;Hou S
紫杉醇是一线抗肿瘤药物，对结直肠癌的疗效较低。与其他药物联合使用是增强紫杉醇细胞毒性的有效策略。康莱特注射剂是薏苡仁的提取物，通常与其他药物合用治疗癌症。本研究旨在探讨紫杉醇联合康莱特对结直肠癌细胞系的治疗作用。康莱特预处理后紫杉醇治疗的协同增效效果最好。这种组合也导致Balb/c小鼠模型中肿瘤体积最小。康莱特抑制核因子(NF)-κΒ的表达，上调连接蛋白43的表达，使癌细胞对紫杉醇敏感。此外，康莱特增加了紫杉醇引起的许多细胞变异，包括微管蛋白聚合、caspase-3裂解、survivin和cyclin B1表达上调。提示康莱特预处理可增强紫杉醇对结直肠癌的治疗作用。
Taxol, a first-line anti-tumour drug, has low effectiveness against colorectal cancer. Combination with other agents is an effective strategy to enhance Taxol cytotoxicity. Kanglaite injection is an extract fromCoix lacryma-jobiseed and is usually combined with other agents to treat cancer. The aim of this study was to investigate the treatment effect of Taxol combined with Kanglaite on colorectal cancer cell lines. Kanglaite pretreatment followed by Taxol treatment was found to show the best synergism among all combination strategies. This combination also resulted in the smallest tumour volume in a Balb/c mice model. Kanglaite inhibited the expression of nuclear factor (NF)-κΒ and upregulated that of connexin 43, both of which sensitized cancer cells to Taxol. Moreover, Kanglaite increased many cellular variations caused by Taxol, including tubulin polymerization, caspase-3 cleavage, and upregulated expression of survivin and cyclin B1. These results suggest that Kanglaite pretreatment may increase the effect of Taxol on colorectal cancer.