Meiotic DNA breaks at the S. pombe recombination hot spot M26.
Meiotic DNA breaks at the S. pombe recombination hot spot M26.
复制标题
DOI：
10.1016/s1097-2765(02)00489-6
复制
发表时间：
2002-04
期刊：
影响因子：
16
通讯作者：
W. Steiner;Randall W. Schreckhise;Gerald R. Smith
中科院分区：
文献类型：
--
作者：
W. Steiner;Randall W. Schreckhise;Gerald R. Smith
粟酒裂殖酵母的ade 6-M26等位基因产生了一个明确的减数分裂重组热点，该热点需要一个特定的序列5′-ATGACGT-3′和Atf 1·Pcr 1转录因子才能发挥活性。我们发现，M26刺激减数分裂特异性双链DNA断裂的形成在多个位点周围的M26。像热点活动，断裂需要theM 26七聚体，Pcr 1，和一般重组因子Rec 12。当将M26七聚体移动到一个新的位置时，在移动的七聚体周围观察到新的断裂位点，其跨度为0.5- 2kb。断裂频率与这些等位基因的重组频率密切相关。M26处断裂的发生表明与远亲酵母酿酒酵母中的热点在机制上相似。
Theade6-M26allele ofSchizosaccharomyces pombecreates a well-defined meiotic recombination hot spot that requires a specific sequence, 5′-ATGACGT-3′, and the Atf1•Pcr1 transcription factor for activity. We find thatM26stimulates the formation of meiosis-specific double-strand DNA breaks at multiple sites surroundingM26. Like hot spot activity, breakage requires theM26heptamer, Pcr1, and the general recombination factor Rec12. When theM26heptamer is moved to new positions withinade6, new break sites are observed spanning ∼0.5–2 kb around the moved heptamer. Break frequency is strongly correlated with recombination frequency for these alleles. The occurrence of breaks atM26suggests mechanistic similarities to hot spots in the distantly related yeastSaccharomyces cerevisiae.