Production of Biodiesel Fuel from the Microalga Schizochytrium limacinum by Direct Transesterification of Algal Biomass
Production of Biodiesel Fuel from the Microalga Schizochytrium limacinum by Direct Transesterification of Algal Biomass
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DOI：
10.1021/ef900704h
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发表时间：
2009-10-01
期刊：
影响因子：
5.3
通讯作者：
Wen, Zhiyou
中科院分区：
文献类型：
--
作者：
Johnson, Michael B.;Wen, Zhiyou
从微藻中生产生物燃料最近重新引起了人们的兴趣。裂殖壶菌（Schizochytriumlimacinum）是一种异养微生物，能够产生高水平的生物量和总脂肪酸。这项工作的目的是探索使用不同的生物柴油制备方法从这种藻类生产生物柴油的潜力，包括油提取然后酯交换（两步法）或藻类生物质的直接酯交换（一步法）。当冷冻干燥的生物质用作原料时，两阶段方法导致粗生物柴油产率（基于藻类生物质）为57%，脂肪酸甲酯（FAME）含量为66.37%。一步法（在酯交换中使用氯仿、己烷或石油醚）导致粗生物柴油的高产率，而仅基于氯仿的酯交换导致高FAME含量。当湿生物质用作原料时，一步法导致生物柴油产率低得多。对通过干生物质的直接酯交换制备的生物柴油进行ASTM标准测试。游离甘油、总甘油、酸值、皂含量、对铜的腐蚀性、闪点、粘度、颗粒物等指标均符合ASTM标准，水分、泥沙含量、硫含量等指标不合格。总的来说，结果表明了这一点。limanicum是通过直接酯交换法生产生物柴油的合适原料。
Producing biofuel from microalgae has gained renewed interest recently. Schizochytrium limacinum is a heterotrophic microalga that is capable of producing high levels of biomass and total fatty acid. The objective of this work is to explore the potential of producing biodiesel fuel from this alga using different biodiesel preparation methods, including oil extraction followed by transesterification (a two-stage method) or direction transesterification of algal biomass (a one-stage method). When freeze-dried biomass was used as feedstock, the two-stage method resulted in 57% of crude biodiesel yield (based on algal biomass) with a fatty acid methyl ester (FAME) content of 66.37%. The one-stage method (with chloroform, hexane, or petroleum ether used in transesterification) led to a high yield of crude biodiesel, whereas only chloroform-based transesterification led to a high FAME content. When wet biomass was used as feedstock, the one-stage method resulted in a much-lower biodiesel yield. The biodiesel prepared via the direct transesterification of dry biomass was subjected to ASTM standard tests. Parameters such as free glycerol, total glycerol, acid number, soap content, corrosiveness to copper, flash point, viscosity, and particulate matter met the ASTM standards, while the water and sediment content, as well as the sulfur content did not pass the standard. Collectively, the results indicate the alga S. limanicum is a suitable feedstock for producing biodiesel via the direct transesterification method.