Expression of neurocan mRNA and ultrastructure of brain tissue after cerebral ischemia and reperfusion in stroke-prone renovascular hypertensive rats treated by electroacupuncture
Expression of neurocan mRNA and ultrastructure of brain tissue after cerebral ischemia and reperfusion in stroke-prone renovascular hypertensive rats treated by electroacupuncture
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电针治疗脑卒中易发性肾血管性高血压大鼠脑缺血再灌注后脑组织neurocan mRNA表达及超微结构
DOI：
10.3969/j.issn.1673-5374.2011.36.006
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发表时间：
2011-12-01
影响因子：
6.1
通讯作者：
Sun, Jingbo
中科院分区：
文献类型：
--
作者：
Tan, Feng;Wan, Saiying;Sun, Jingbo
采用银环夹缩双侧肾动脉的方法建立易卒中型肾血管性高血压大鼠模型。10周后，诱导大脑中动脉闭塞2小时。缺血30天后电针百会、大椎穴。原位杂交结果显示，电针可明显减少缺血半暗带和海马组织中神经肽mRNA阳性细胞的数量。电镜结果显示电针可使缺血组织神经元和血管结构得到恢复。总之，这些数据表明，电针可以保护神经元免受缺血再灌注损伤在易卒中肾血管性高血压大鼠，这可能是通过下调神经抑制因子neurocan mRNA的表达。
We established a stroke-prone renovascular hypertensive rat model by bilateral constriction of the renal artery with sliver loop clips. After ten weeks, middle cerebral artery occlusion was induced for 2 hours. The rats then received electro-acupuncture at Baihui (DU 20) and Dazhui (DU 14) after onset of ischemia for 30 days. In situ hybridization study showed that electroacupuncture significantly reduced the number of neurocan mRNA-positive cells in the ischemic penumbra and hippocampal tissues of rats. Electron microscopy results demonstrated that the structure of neurons and blood vessels in the ischemic tissues were restored with electroacupuncture. Overall, these data suggest that electroacupuncture may protect neurons against ischemic reperfusion injury in stroke-prone renovascular hypertensive rats, which may be regulated by downregulation of expression of nerve inhibitory factor neurocan mRNA.