Cyst fluid hemoglobin species in endometriosis and its malignant transformation: The role of metallobiology
Cyst fluid hemoglobin species in endometriosis and its malignant transformation: The role of metallobiology
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DOI：
10.3892/ol.2016.4383
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发表时间：
2016-05-01
期刊：
影响因子：
2.9
通讯作者：
Kobayashi, Hiroshi
中科院分区：
文献类型：
--
作者：
Iwabuchi, Takuya;Yoshimoto, Chiharu;Kobayashi, Hiroshi
本研究的目的是评估血红蛋白（Hb）的相对浓度在子宫内膜异位症作为恶性肿瘤的可能指标。采用电子吸收光谱法对收集自8例卵巢癌相关性囊肿（EAOC）患者的囊液中存在的Hb种类进行定量，并与35例良性卵巢囊肿患者的囊液中存在的Hb种类进行比较。电子吸收光谱中的620/580 nm比值用作高铁血红蛋白（metHb）/（氧合血红蛋白+metHb）比值的替代指标，通过紫外/可见光光栅分光光度酶标仪测量每个囊液中的620/580 nm比值。根据受试者工作特征（ROC）曲线确定最佳截断值。根据EAOC与子宫内膜异位症的临界值计算检测的敏感性和特异性。EAOC患者的囊液620/580 nm比值远低于良性囊肿患者（0.389 +/- 0.266 vs. 0.666 +/- 0.188，P=0.021）。以0.35为最佳临界值进行ROC曲线分析，结果表明620/580 nm比值诊断EAOC的敏感性、特异性、阳性预测值（PPV）和阴性预测值分别为62.5%、100.0%、100.0%和92.1%。总之，metHb是良性囊肿中最丰富的Hb种类之一，囊液的620/580 nm吸收比具有较高的特异性和PPV作为监测试验，用于早期检测子宫内膜异位症恶变。因此，金属生物学突出了血红蛋白稳态和子宫内膜异位症恶变的发病机制所涉及的不同特征。
The aim of the present study was to assess the relative concentrations of hemoglobin (Hb) species in endometriosis as a possible indicator of malignancy. Electronic absorption spectroscopy was employed to quantify the Hb species present in the cyst fluid collected from 8 patients with endometriosis-associated ovarian cancer (EAOC), and compared with those present in the cyst fluid of 35 patients with benign endometriotic cysts. The 620/580 nm ratio in the electronic absorption spectrum, which was used as a surrogate indicator of the methemoglobin (metHb)/(oxyhemoglobin+metHb) ratio, was measured in each cyst fluid by ultraviolet/visible grating spectrophotometric microplate reader. The optimal cutoff value was defined according to the analysis of receiver operating characteristic (ROC) curve. The sensitivity and specificity of detection were calculated on the basis of the cutoff value to differentiate EAOC from endometriosis. The 620/580 nm ratio of cyst fluid in EAOC patients was much lower than that measured in women with benign cysts (0.389 +/- 0.266 vs. 0.666 +/- 0.188, P=0.021). ROC curve analysis performed using 0.35 as the optimal cutoff value indicated that the 620/580 nm ratio had a sensitivity, specificity, positive predictive value (PPV) and negative predictive value of 62.5, 100.0, 100.0 and 92.1%, respectively, in the diagnosis of EAOC. In conclusion, metHb is one of the most abundant Hb species in benign cysts, and the absorption 620/580 nm ratio of cyst fluid exhibits high specificity and PPV as a surveillance test for the early detection of malignant transformation of endometriosis. Thus, metallobiology highlights diverse features involved in Hb homeostasis and the pathogenesis of malignant transformation of endometriosis.