PHYSIOLOGICAL CONSTRAINTS IN INCREASING BIOMASS CONCENTRATION OF ESCHERICHIA-COLI-B IN FED-BATCH CULTURE
PHYSIOLOGICAL CONSTRAINTS IN INCREASING BIOMASS CONCENTRATION OF ESCHERICHIA-COLI-B IN FED-BATCH CULTURE
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DOI：
10.1007/bf01032744
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发表时间：
1987-02-01
影响因子：
2.7
通讯作者：
LEBEAULT, JM
中科院分区：
文献类型：
--
作者：
PAN, JG;RHEE, JS;LEBEAULT, JM
对大肠杆菌B进行补料分批培养以获得高浓度的生物量。通过喷射纯氧满足氧气需求后，描述了生理限制。高CO2分压导致最大比生长率下降，而发酵副产物则导致生物量产量和最大比生长率下降。
Fed-batch culture of E. coli B was carried out to obtain high concentration of biomass. After requirement of oxygen was met by sparging the pure oxygen, physiological constraints were delineated. High partial pressure of CO2 caused the decrease of the maximum specific growth rate, whereas fermentative byproducts caused the decrease of biomass yield as well as the maximum specific growth rate.