Characteristics of Electrospray-Ionization Detection of Synthetic Di- to Penta-Oligopeptides by Amine Derivatizations
Characteristics of Electrospray-Ionization Detection of Synthetic Di- to Penta-Oligopeptides by Amine Derivatizations
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胺衍生法电喷雾电离检测合成二至五寡肽的特点
DOI：
10.2116/analsci.21n016
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发表时间：
2021-05
影响因子：
1.6
通讯作者：
Weilin Shen;Kiyomi Ono;Mitsuru Tanaka;T. Matsui
中科院分区：
文献类型：
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作者：
Weilin Shen;Kiyomi Ono;Mitsuru Tanaka;T. Matsui
化学衍生化已广泛用于生物活性小肽的高灵敏度检测；然而，从较长寡肽的角度来看，它们的优势尚未得到证实。在本研究中，通过四种胺衍生方法对由甘氨酸和肌氨酸组成的合成二肽至五肽进行电喷雾电离（ESI）-质谱（MS）检测。结论是，二肽至五肽的 ESI-MS 检测以衍生肽部分的分子表面积为特征，最佳值为 250–300 Å2，无论疏水性和衍生方法如何。
Chemical derivatizations have been extensively developed for highly sensitive detection of bioactive small peptides; however, their advantages from the viewpoint of longer oligopeptides remain unverified. In this study, electrosprayionization (ESI)-mass spectrometric (MS) detection of synthetic di- to pentapeptides consisting of glycine and sarcosine were characterized by four amine derivatization methods. It was concluded that the ESI-MS detection of di- to pentapeptides was characterized by the molecular surface area of derivatized peptide moieties with an optimal value of 250–300 Å2, regardless of hydrophobicity and derivatization methods.