A novel gene iba1 in the major histocompatibility complex class III region encoding an EF hand protein expressed in a monocytic lineage
A novel gene iba1 in the major histocompatibility complex class III region encoding an EF hand protein expressed in a monocytic lineage
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DOI：
10.1006/bbrc.1996.1112
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发表时间：
1996-07-25
影响因子：
3.1
通讯作者：
Kohsaka, S
中科院分区：
文献类型：
--
作者：
Imai, Y;Ibata, I;Kohsaka, S
我们成功地分离出一个大鼠cDNA克隆编码一种新的EF手蛋白的分子量约为17 kDa，并指定此基因iba1（离子钙结合衔接分子1）。iba1基因的基因组拷贝位于Bat2和TNF α基因之间的主要组织相容性复合体III类区域的片段内。iba1基因在睾丸和脾脏中高表达，而在脑、肺和肾中弱表达。在脑细胞中，iba1基因在小胶质细胞中特异性表达。造血细胞系的筛选显示，Iba1蛋白在单核细胞系中明确表达，但在髓系细胞系中仅非常弱地表达。因此，Ibal蛋白被认为是一个衔接分子，介导钙信号，可能在单核细胞谱系，包括小胶质细胞的功能。(C)出版社：Academic Press，Inc.
We successfully isolated a rat cDNA clone encoding a novel EF hand protein with a molecular weight of about 17 kDa and designated this gene iba1 (ionized calcium binding adapter molecule 1). The genomic copy of the iba1 gene was located within a segment of the major histocompatibility complex class III region between the Bat2 and TNF alpha genes. The iba1 gene was shown to be highly expressed in testis and spleen, but weakly expressed in brain, lung, and kidney. Among brain cells, the iba1 gene was specifically expressed in microglia. A screening of hemopoietic cell lines showed that the Iba1 protein was clearly expressed in monoblastic cell lines but only very weakly expressed in myeloid cell lines. Ibal protein is therefore suggested to act as an adapter molecule, mediating calcium signals that may function in a monocytic lineage including microglia. (C) 1996 Academic Press, Inc.