Longitudinal intravital imaging of mouse placenta
Longitudinal intravital imaging of mouse placenta
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DOI：
10.1126/sciadv.adk1278
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发表时间：
2024-03-20
期刊：
影响因子：
13.6
通讯作者：
Yao，Junjie
中科院分区：
文献类型：
--
作者：
Zhu，Xiaoyi;Huang，Qiang;Yao，Junjie
研究胎盘功能对于了解妊娠并发症至关重要。然而，由于其深度、体积和运动失真，胎盘成像具有挑战性。在这项研究中，我们开发了一种小鼠植入式胎盘窗，可以在整个妊娠期间对胎盘发育进行高分辨率光声和荧光成像。胎盘窗对光线和声音具有极好的透明度。通过将胎盘窗口与超快功能光声显微镜相结合，我们能够研究整个小鼠妊娠期间的胎盘发育，提供前所未有的时空细节。因此，我们研究了胎盘对饮酒和心脏骤停的急性反应，以及炎症模型中的慢性异常。我们还观察到病毒基因传递在单细胞水平和化学扩散通过胎盘使用荧光成像。我们的研究结果表明，通过胎盘窗的活体成像可以成为研究胎盘功能和了解不良妊娠结局的胎盘起源的有力工具。
Studying placental functions is crucial for understanding pregnancy complications. However, imaging placenta is challenging due to its depth, volume, and motion distortions. In this study, we have developed an implantable placenta window in mice that enables high-resolution photoacoustic and fluorescence imaging of placental development throughout the pregnancy. The placenta window exhibits excellent transparency for light and sound. By combining the placenta window with ultrafast functional photoacoustic microscopy, we were able to investigate the placental development during the entire mouse pregnancy, providing unprecedented spatiotemporal details. Consequently, we examined the acute responses of the placenta to alcohol consumption and cardiac arrest, as well as chronic abnormalities in an inflammation model. We have also observed viral gene delivery at the single-cell level and chemical diffusion through the placenta by using fluorescence imaging. Our results demonstrate that intravital imaging through the placenta window can be a powerful tool for studying placenta functions and understanding the placental origins of adverse pregnancy outcomes.