Intracellular survival and replication of Vibrio cholerae O139 in aquatic free-living amoebae
Intracellular survival and replication of Vibrio cholerae O139 in aquatic free-living amoebae
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DOI：
10.1111/j.1462-2920.2005.00771.x
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发表时间：
2005-07-01
影响因子：
5.1
通讯作者：
Sandström, G
中科院分区：
文献类型：
--
作者：
Abd, H;Weintraub, A;Sandström, G
霍乱弧菌是一种高度传染性的细菌，定期在人类中造成霍乱的大规模爆发。流行病的季节性分布是已知的，但自然栖息地的作用几乎未知。浮游生物被认为发挥了一定作用，因为细菌可以附着在这些生物体上形成生物膜。卡氏棘阿米巴是一种环境阿米巴原虫，已被证明能够摄入并促进多种不同来源的细菌生长。本研究的目的是确定霍乱弧菌 O139 是否存在阿米巴内行为。通过可培养计数、庆大霉素测定、电子显微镜和聚合酶链式反应研究了共培养中这些微生物之间的相互作用。这种相互作用导致霍乱弧菌在阿米巴内生长并在滋养体细胞质以及卡斯氏阿米巴包囊中存活。这些数据表明这些微生物之间存在共生关系，霍乱弧菌的兼性细胞内行为与普遍观点相矛盾，以及自由生活的变形虫作为霍乱弧菌 O139 宿主的作用。总而言之，这为研究霍乱的生态学、免疫学、流行病学和治疗打开了新的大门。
Vibrio cholerae is a highly infectious bacterium responsible for large outbreaks of cholera among humans at regular intervals. A seasonal distribution of epidemics is known but the role of naturally occurring habitats are virtually unknown. Plankton has been suggested to play a role, because bacteria can attach to such organisms forming a biofilm. Acanthamoebea castellanii is an environmental amoeba that has been shown to be able to ingest and promote growth of several bacteria of different origin. The aim of the present study was to determine whether or not an intra-amoebic behaviour of V. cholerae O139 exists. Interaction between these microorganisms in co-culture was studied by culturable counts, gentamicin assay, electron microscopy, and polymerase chain reaction. The interaction resulted in intra-amoebic growth and survival of V. cholerae in the cytoplasm of trophozoites as well as in the cysts of A. castellanii. These data show symbiosis between these microorganisms, a facultative intracellular behaviour of V. cholerae contradicting the generally held view, and a role of free-living amoebae as hosts for V. cholerae O139. Taken together, this opens new doors to study the ecology, immunity, epidemiology, and treatment of cholera.