New evidence for nitrogen fixation within the Italian white truffle Tuber magnatum
New evidence for nitrogen fixation within the Italian white truffle Tuber magnatum
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DOI：
10.1016/j.funbio.2010.09.001
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发表时间：
2010-11-01
期刊：
影响因子：
2.5
通讯作者：
Stocchi, Vilberto
中科院分区：
文献类型：
--
作者：
Barbieri, Elena;Ceccaroli, Paola;Stocchi, Vilberto
研究了意大利白色块菌中固氮菌的多样性及其固氮活性。利用简并PCR引物扩增出固氮酶基因nifH。不同成熟阶段的Magnatum ascomata。推测的氨基酸序列主要揭示了属于慢生根瘤菌属的Alphaproteobacteria的存在。并检测了慢生根瘤菌nifH基因的表达。用乙炔还原法测得的固氮酶活性为0.5-7.5 μ mol C（2）H（4）h（-1）g（-1），与豆科植物早期根瘤中的特定固氮菌相当。这是首次在块菌中证实固氮酶的表达基因和活性。(C)2010年英国真菌学会。由爱思唯尔有限公司出版。保留所有权利。
Diversity of nitrogen-fixing bacteria and the nitrogen-fixation activity was investigated in Tuber magnatum, the most well-known prized species of Italian white truffle. Degenerate PCR primers were applied to amplify the nitrogenase gene nifH from T. magnatum ascomata at different stages of maturation. Putative amino acid sequences revealed mainly the presence of Alphaproteobacteria belonging to Bradyrhizobium spp. and expression of nifH genes from Bradyrhizobia was detected. The nitrogenase activity evaluated by acetylene reduction assay was 0.5-7.5 mu mol C(2)H(4)h(-1)g(-1), comparable with early nodules of legumes associated with specific nitrogen-fixing bacteria. This is the first demonstration of nitrogenase expression gene and activity within truffle. (C) 2010 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.