Genetic Control of Tryptophan Peroxidase-Oxidase in Drosophila melanogaster
Genetic Control of Tryptophan Peroxidase-Oxidase in Drosophila melanogaster
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DOI：
10.1038/1841084a0
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发表时间：
1959-10
期刊：
影响因子：
64.8
通讯作者：
C. Baglioni
中科院分区：
文献类型：
--
作者：
C. Baglioni
BUTENANDT 1、Beadle 2和Ephrussi 3已经表明，犬尿氨酸的合成发生在昆虫中，而在黑腹果蝇中，基因“vermillion”（ν）控制犬尿氨酸的形成。Knox和Mehler 4在哺乳动物肝脏中广泛研究了色氨酸氧化为犬尿氨酸，他们发现两种酶参与了这两步反应：色氨酸过氧化物酶-氧化酶和犬尿氨酸甲酰胺酶。由于突变体ν含有正常量的后一种酶5，人们认为色氨酸氧化的第一步（导致甲酰犬尿氨酸）在某种程度上受到这种突变的影响。到目前为止，还没有人直接证明ν基因对酶的控制;工作人员试图证明色氨酸是通过果蝇提取物在体外代谢的，但没有成功。色氨酸过氧化物酶-氧化酶的含量很低和酶测定方法的相对不足可能是导致这些结果的原因。直到最近才在一种昆虫--食蛾Ephestia kuhniella 7中发现色氨酸过氧化物酶-氧化酶的活性，并通过测量色谱分离斑点的荧光进行定量测定。本文报道了用一种非常灵敏的方法研究黑腹果蝇中色氨酸过氧化物酶氧化酶的遗传控制。
BUTENANDT1, Beadle2and Ephrussi3have shown that kynurenine synthesis occurs in insects and that inD. melanogasterthe gene ‘vermillion’ (ν) controls kynurenine formation. Tryptophan oxidation to kynurenine was extensively studied in mammal liver by Knox and Mehler4, who showed that two enzymes are involved in this two-step reaction: tryptophan peroxidase-oxidase and kynurenine formamidase. Since the mutantνcontains a normal amount of the latter enzyme5, it was thought that the first step of tryptophan oxidation, which leads to formylkynurenine, is in some way affected by this mutation. No direct demonstration of enzyme control by theνgene has been given up to now; workers have tried unsuccessfully to demonstrate that tryptophan is metabolizedin vitrobyDrosophilaextracts6. A very low content of tryptophan peroxidase-oxidase and the relative inadequacy of the methods of enzyme assay, account probably for these results. Only recently activity of tryptophan peroxidase-oxidase was recognized in an insect—the meal-mothEphestia kuhniella7, and quantitative determinations were made by measuring the fluorescence of chromatographically separated spots. This communication deals with the genetic control of tryptophan peroxidase oxidase inD. melanogasterstudied by means of a very sensitive method.