Effects of ink characteristics and piezo-electric inkjetting parameters on lysozyme activity
Effects of ink characteristics and piezo-electric inkjetting parameters on lysozyme activity
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DOI：
10.1038/s41598-019-54723-9
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发表时间：
2019-12-03
影响因子：
4.6
通讯作者：
Nierstrasz, Vincent A.
中科院分区：
文献类型：
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作者：
Biswas, Tuser T.;Yu, Junchun;Nierstrasz, Vincent A.
酶的喷墨打印可以促进许多新的应用，其中少量材料需要以精确和灵活的方式沉积。然而，由于油墨流变性和打印头参数的要求，保持喷墨打印酶的令人满意的活性是一项具有挑战性的任务。因此，为了找到最佳的喷墨条件，我们研究了几种墨水配方和喷射参数对溶菌酶活性的影响，使用压电打印头。在蛋白质浓度的线性活性范围内，含50 μ g/mL溶菌酶的墨水显示出令人满意的85%的活性保留。在pH 6.2和0.06摩尔的离子强度下发现喷射的油墨的可接受的活性。甘油被发现是一种有效的粘度调节剂（10-15 mPa.s），保湿剂和蛋白质结构稳定剂的制备油墨。一种非离子表面活性剂，当使用低于临界胶束浓度被发现是有利的喷射油墨。对于在室温温育24小时后喷射的油墨，观察到活性保留的增加。然而，对于高于室温的喷墨没有观察到额外的活性。这项研究的结果将有助于制定其他蛋白质为基础的油墨和设置其喷墨打印参数，而不会高度损害的功能。
Inkjet printing of enzymes can facilitate many novel applications where a small amount of materials need to be deposited in a precise and flexible manner. However, maintaining the satisfactory activity of inkjet printed enzyme is a challenging task due to the requirements of ink rheology and printhead parameters. Thus to find optimum inkjetting conditions we studied the effects of several ink formulation and jetting parameters on lysozyme activity using a piezoelectric printhead. Within linear activity range of protein concentrations ink containing 50 mu g/mL lysozyme showed a satisfactory activity retention of 85%. An acceptable activity of jetted ink was found at pH 6.2 and ionic strength of 0.06 molar. Glycerol was found to be an effective viscosity modifier (10-15 mPa.s), humectant and protein structure stabilizer for the prepared ink. A non-ionic surfactant when used just below critical micelle concentration was found to be favourable for the jetted inks. An increase in activity retention was observed for inks jetted after 24 hours of room temperature incubation. However, no additional activity was seen for inkjetting above the room temperature. Findings of this study would be useful for formulating other protein-based inks and setting their inkjet printing parameters without highly compromising the functionality.