Elevated expression of eukaryotic translation initiation factor 3H is associated with proliferation, invasion and tumorigenicity in human hepatocellular carcinoma.
Elevated expression of eukaryotic translation initiation factor 3H is associated with proliferation, invasion and tumorigenicity in human hepatocellular carcinoma.
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真核翻译起始因子 3H 的表达升高与人肝细胞癌的增殖、侵袭和致瘤性相关。
DOI：
10.18632/oncotarget.10222
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发表时间：
2016-08-02
期刊：
影响因子：
--
通讯作者：
Du ZY
中科院分区：
文献类型：
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作者：
Zhu Q;Qiao GL;Zeng XC;Li Y;Yan JJ;Duan R;Du ZY
我们研究了真核生物翻译起始因子3亚基H（EIF 3 H）在肝细胞癌（HCC）进展中的作用。50.23%的患者EIF 3 H高表达。EIF 3 H的上调是HCC患者癌症复发率较高和总生存期较短的独立预测因子。在HCC细胞中敲低EIF 3 H表达促进凋亡，并抑制细胞生长、集落形成、迁移以及异种移植物生长。TGF-β和MAPK通路可能是EIF 3 H的靶向通路。在HCC组织样品和配对的非肿瘤样品（N=60）中测量EIF 3 H mRNA表达，并在215名HCC患者的另一数据集中验证结果。EIF 3 H表达与临床预后相关。在HCC细胞系中用siRNA敲低EIF 3 H表达后研究恶性表型。通过微阵列分析鉴定EIF 3 H靶向通路。EIF 3 H经常上调，是HCC患者的独立预后标志物，EIF 3 H抑制可减轻恶性表型。我们的数据为EIF 3 H在HCC进展中的功能提供了新的见解，并表明EIF 3 H可能是HCC的潜在有价值的生物标志物。
We studied the role of eukaryotic translation initiation factor 3 subunit H (EIF3H) in hepatocellular carcinoma (HCC) progression. High EIF3H expression was observed in 50.23% patients. Upregulation of EIF3H is an independent predictor for greater rates of cancer recurrence and shorter overall survival in HCC patients. Knockdown of EIF3H expression in HCC cells promoted apoptosis, and inhibited cell growth, colony formation, migration, as well as xenograft growth. TGF-βand MAPK pathways are potentially targeted by EIF3H. EIF3H mRNA expression was measured in HCC tissue samples and paired non-tumor samples (N=60) and results were validated in another dataset of 215 HCC patients. Then EIF3H expression and clinical outcomes were correlated. Malignant phenotypes were studied after EIF3H expression was knocked down with siRNA in HCC cell lines. EIF3H targeted pathways were identified by microarray analysis. EIF3H is frequently upregulated and is an independent prognostic marker for HCC patients and EIF3H inhibition mitigates the malignant phenotype. Our data provide novel insight into the function of EIF3H in HCC progression, and suggest that EIF3H may be a potentially valuable biomarker for HCC.