NMDA Receptor Enhances Correlation of Spontaneous Activity in Neonatal Barrel Cortex
NMDA Receptor Enhances Correlation of Spontaneous Activity in Neonatal Barrel Cortex
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DOI：
10.1523/jneurosci.0527-20.2020
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发表时间：
2021-02-10
影响因子：
5.3
通讯作者：
Iwasato, Takuji
中科院分区：
文献类型：
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作者：
Mizuno, Hidenobu;Rao, Madhura S.;Iwasato, Takuji
相关自发活动在发育过程中新皮层回路的组织中起着关键作用。然而，调节活动相关性的皮质机制仍然是难以捉摸的。在这项研究中，使用双光子钙成像的桶皮质层4（L4）在生活的新生小鼠，我们发现，NMDA受体（NMDARs）在L4神经元的自发活动相关性的增强是重要的。破坏GluN1（Grin1），一个强制性的NMDAR亚基，在稀疏的L4神经元群体减少活动之间的相关性GluN1敲除（GluN1KO）神经元对桶内。甚至在邻近桶的L4神经元对中也检测到了这种活动相关性的降低，并且当一个或两个神经元位于桶边缘时最为明显。我们的研究结果提供了证据，参与L4神经元NMDAR的空间组织的自发放电活动的L4神经元在新生儿桶皮质。
Correlated spontaneous activity plays critical role in the organization of neocortical circuits during development. However, cortical mechanisms regulating activity correlation are still elusive. In this study, using two-photon calcium imaging of the barrel cortex layer 4 (L4) in living neonatal mice, we found that NMDA receptors (NMDARs) in L4 neurons are important for enhancement of spontaneous activity correlation. Disruption of GluN1 (Grin1), an obligatory NMDAR subunit, in a sparse population of L4 neurons reduced activity correlation between GluN1 knock-out (GluN1KO) neuron pairs within a barrel. This reduction in activity correlation was even detected in L4 neuron pairs in neighboring barrels and most evident when either or both of neurons are located on the barrel edge. Our results provide evidence for the involvement of L4 neuron NMDARs in spatial organization of the spontaneous firing activity of L4 neurons in the neonatal barrel cortex.