Organisation of afferents along the anterior-posterior and medial-lateral axes of the rat orbitofrontal cortex
Organisation of afferents along the anterior-posterior and medial-lateral axes of the rat orbitofrontal cortex
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沿着大鼠眶额皮质的前后轴和内侧轴的传入组织
DOI：
10.1101/2020.08.28.272591
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Barreiros I
中科院分区：
文献类型：
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作者：
Barreiros I
眶额皮质（orbitofrontal cortex，OFC）在解剖学上沿其内侧-外侧轴沿着可分为多个亚区，行为学研究表明这些亚区具有不同的功能。最近，有证据表明，功能多样性也存在沿着啮齿动物眶额肌的喙尾轴。然而，这些差异背后的解剖学连接模式尚未得到很好的表征。在这里，我们使用的逆行示踪剂霍乱毒素亚基B，同时标记的预测到前外侧（ALO）和后外侧（PLO），和后腹侧（PVO）部分的大鼠OFC。我们的方法允许我们同时比较这些OFC细分的密度和输入模式。我们观察到不同的和地形组织的投影模式到ALO，PLO和PVO从mediaodorsal和submedius核的丘脑。我们还观察到来自杏仁核、运动皮质、感觉皮质和内侧前额叶皮质结构（包括内侧OFC、边缘下皮质和边缘前皮质）的这些OFC分区的不同水平的连接强度。有趣的是，虽然在这些输入区域中的一些标签只显示了连接强度的梯度，但其他区域似乎几乎完全投射到特定的OFC细分。此外，ALO和PLO之间的输入模式的差异与PLO和PVO之间的差异一样明显。总之，我们的研究结果支持存在不同的解剖电路内外侧OFC沿着其前后轴。
The orbitofrontal cortex (OFC) has been anatomically divided into a number of subregions along its medial-lateral axis, which behavioural research suggests have distinct functions. Recently, evidence has emerged suggesting functional diversity is also present along the rostral-caudal axis of the rodent OFC. However, the patterns of anatomical connections that underlie these differences have not been well characterised. Here, we use the retrograde tracer cholera toxin subunit B to simultaneously label the projections into the anterior lateral (ALO) and posterior lateral (PLO), and posterior ventral (PVO) portions of the rat OFC. Our methodological approach allowed to us to simultaneously compare the density and input patterns into these OFC subdivisions. We observed distinct and topographically organised projection patterns into ALO, PLO and PVO from the mediodorsal and the submedius nuclei of the thalamus. We also observed different levels of connectivity strength into these OFC subdivisions from the amygdala, motor cortex, sensory cortices and medial prefrontal cortical structures, including medial OFC, infralimbic and prelimbic cortices. Interestingly, while labelling in some of these input regions revealed only a gradient in connectivity strength, other regions seem to project almost exclusively to specific OFC subdivisions. Moreover, differences in input patterns between ALO and PLO were as pronounced as those between PLO and PVO. Together, our results support the existence of distinct anatomical circuits within lateral OFC along its anterior-posterior axis.