Glutathione peroxidase 2 in Saccharomyces cerevisiae is distributed in mitochondria and involved in sporulation.
Glutathione peroxidase 2 in Saccharomyces cerevisiae is distributed in mitochondria and involved in sporulation.
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DOI：
10.1016/j.bbrc.2011.06.189
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发表时间：
2011-08
影响因子：
3.1
通讯作者：
Yuuta Ukai;T. Kishimoto;T. Ohdate;S. Izawa;Y. Inoue
中科院分区：
文献类型：
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作者：
Yuuta Ukai;T. Kishimoto;T. Ohdate;S. Izawa;Y. Inoue
Gpx2是酿酒酵母中谷胱甘肽过氧化物酶的三个同源物之一，是一种非典型的2-半胱氨酸过氧化还蛋白，在体外更倾向于使用硫氧还蛋白作为还原剂。尽管Gpx2是一种抗氧化剂，但尚未发现gpx2Δ突变细胞在氧化应激方面的明显表型。为了了解Gpx2‘S在体内的生理功能，我们确定了它在细胞内的分布。Gpx2存在于细胞质和线粒体中。在线粒体中，Gpx2与胞质侧的外膜和基质侧的内膜相连。线粒体Gpx2的氧化还原状态受Trx1和Trx2(细胞质硫氧还蛋白)和Trx3(线粒体基质硫氧还蛋白)的调节。此外，我们还发现GPX2的破坏降低了二倍体细胞的产孢率。
Gpx2, one of three glutathione peroxidase homologs (Gpx1, Gpx2, and Gpx3) in Saccharomyces cerevisiae, is an atypical 2-Cys peroxiredoxin that prefers to use thioredoxin as a reducing agent in vitro. Despite Gpx2 being an antioxidant, no obvious phenotype of gpx2Δ mutant cells in terms of oxidative stress has yet been found. To gain a clue as to Gpx2’s physiological function in vivo, here we identify its intracellular distribution. Gpx2 was found to exist in the cytoplasm and mitochondria. In mitochondria, Gpx2 was associated with the outer membrane of the cytoplasmic-side, as well as the inner membrane of the matrix-side. The redox state of the mitochondrial Gpx2 was regulated by Trx1 and Trx2 (cytoplasmic thioredoxin), and by Trx3 (mitochondrial matrix thioredoxin). In addition, we found that the disruption of GPX2 reduced the sporulation efficiency of diploid cells.