An expansion phase precedes terminal erythroid differentiation of hematopoietic progenitor cells from cord blood in vitro and is associated with up-regulation of cyclin E and cyclin-dependent kinase 2
An expansion phase precedes terminal erythroid differentiation of hematopoietic progenitor cells from cord blood in vitro and is associated with up-regulation of cyclin E and cyclin-dependent kinase 2
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DOI：
10.1182/blood.v96.12.3985.h8003985_3985_3987
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发表时间：
2000-12-01
期刊：
影响因子：
20.3
通讯作者：
Lu, L
中科院分区：
文献类型：
--
作者：
Dai, MS;Mantel, CR;Lu, L
研究了CD34(+)细胞体外红系增殖和分化过程中细胞周期调控的动态变化。在白介素3(IL-3)、粒细胞-巨噬细胞集落刺激因子(GM-CSF)、钢铁因子和EPO刺激的CD34(+)细胞体外培养7天后，观察到一种异常的细胞周期变化，大多数细胞处于S期(70.2%)，少数细胞处于G1期(27.4%)。红系终末分化伴随着G0/G1期细胞的迅速增加。细胞周期蛋白E和细胞周期蛋白依赖性激酶2的表达与S期细胞比例相关。细胞周期蛋白D3在增殖期呈中度上调，而在终末分化阶段则迅速下调。这表明红细胞的高增殖潜能与细胞周期蛋白E和CDK2的瞬时上调有关。(C)2000年，由美国血液病学会提供。
The dynamics of cell cycle regulation were investigated during in vitro erythroid proliferation and differentiation of CD34(+) cord blood cells, An unusual cell cycle profile with a majority of cells in S phase (70.2%) and minority of cells in G1 phase (27.4%) was observed in burst-forming unit-erythrocytes (BFU-E)-derived erythroblasts from a 7-day culture of CD34(+) cells stimulated with interleukin 3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), Steel factor, and Epo. Terminal erythroid differentiation was accompanied by a rapid increase of G0/G1 phase cells. Expression of cyclin E and cyclin-dependent kinase 2 (cdk2) correlated with the proportion of S phase cells. Cyclin D3 was moderately up-regulated during the proliferation phase, and both cyclin E and D3 were rapidly down-regulated during terminal differentiation. This suggests that the high proliferation potential of erythroblasts is associated with temporal up-regulation of cyclin E and cdk2. (C) 2000 by The American Society of Hematology.