Mouse glandular kallikrein genes. Nucleotide sequence of cloned cDNA coding for a member of the kallikrein arginyl esteropeptidase group of serine proteases.
Mouse glandular kallikrein genes. Nucleotide sequence of cloned cDNA coding for a member of the kallikrein arginyl esteropeptidase group of serine proteases.
复制标题
小鼠腺激肽释放酶基因。
DOI：
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发表时间：
1982
影响因子：
4.8
通讯作者：
J. Shine
中科院分区：
文献类型：
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作者：
R. Richards;D. Catanzaro;A. Mason;B. Morris;J. Baxter;J. Shine
以质粒pBR 322为载体构建了雄性小鼠颌下腺含poly（A）RNA的cDNA克隆文库。通过与放射性标记的cDNA杂交鉴定估计在1-5%丰度级别的含DNA序列，并通过核苷酸序列分析进行检查。通过计算机搜索程序鉴定了编码与迄今为止报道的唯一完整的激肽释放酶序列（来自猪胰腺）具有57%同源性的肽的序列。该插入物似乎编码蛋白质的COOH末端149个氨基酸，因此推测为丝氨酸蛋白酶。该蛋白质的预测氨基酸序列与小鼠颌下腺激肽释放酶酯肽酶组的酶的三个特征性成员中的类似序列的比较揭示了广泛的同源性，但不完全相同。因此，有至少四个成员的这种酶家族中表达的小鼠颌下腺。
A library of cloned cDNA to male mouse submaxillary gland poly(A)-containing RNA was constructed in the plasmid pBR322. Inserts containing sequences estimated to be in the 1-5% abundance class were identified by hybridization to radiolabeled cDNA and examined by nucleotide sequence analysis. A sequence coding for a peptide with 57% homology to the only complete kallikrein sequence reported to date (from pig pancreas) was identified by a computer search program. This insert appears to code for the COOH-terminal 149 amino acids of a protein presumed therefore to be a serine protease. Comparison of the predicted amino acid sequence of this protein with analogous sequences in the three characterized members of the mouse submaxillary gland kallikrein arginyl esteropeptidase group of enzymes revealed extensive homology, although not complete identity. Thus, there are at least four members of this enzyme family expressed in the mouse submaxillary gland.