A comparative study of cell renewal after exposure to ozone or oxygen. Response of terminal bronchiolar epithelium in the rat.
A comparative study of cell renewal after exposure to ozone or oxygen. Response of terminal bronchiolar epithelium in the rat.
复制标题
暴露于臭氧或氧气后细胞更新的比较研究。
DOI：
10.1164/arrd.1978.118.2.335
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
W. Tyler
中科院分区：
文献类型：
--
作者：
H. Lum;L. Schwartz;D. Dungworth;W. Tyler
细胞更新模式的终端细支气管上皮细胞的大鼠进行了检查，在7天的曝光，无论是0.8 ppm的臭氧或80%的O2。将末端细支气管上皮细胞分类并计数为纤毛、非纤毛分泌型（Clara）和非纤毛、非分泌型。使用氢-3标记的胸苷结合光学显微镜放射自显影术，以获得暴露大鼠在6、24、72和168小时的标记指数，并与对照大鼠在6和168小时的标记指数进行比较。结果表明，臭氧和O2都启动了终端细支气管上皮细胞的增殖反应，但对O2损伤的反应延迟发作和较小的幅度。最重要的是，差异细胞计数显示，无纤毛分泌细胞的主要增殖细胞类型在氧化损伤后的所有时期。这种增殖反应持续到168小时。暴露过程中细胞类型和标记指数的比例变化支持了这样的观点，即无纤毛分泌细胞是修复性细支气管细胞，新的纤毛细胞主要来自它们。
Cell renewal patterns of the terminal bronchiolar epithelium of rats were examined during 7-day exposures to either 0.8 ppm of ozone or 80 per cent O2. Terminal bronchiolar epithelial cells were classified and counted in ciliated, nonciliated secretory (Clara), and nonciliated, nonsecretory categories. Thymidine labeled with hydrogen-3 was used in conjunction with light microscopic autoradiography to obtain labeling indices in exposed rats at 6, 24, 72, and 168 hours compared to control rats at 6 and 168 hours. Results indicated that both ozone and O2 initiated a proliferative response of terminal bronchiolar epithelium, but that the response to O2 insult was delayed in onset and of lesser magnitude. Most importantly, the differential cell counts revealed that nonciliated secretory cells were the primary proliferating cell type at all periods after oxidant insult. This proliferative response continued with persistence of the insult through 168 hours. Shifts in proportions of cell types and labeling indices during exposure supported the view that nonciliated secretory cells are the reparative bronchiolar cells and that new ciliated cells are mainly derived from them.