Characterization of a novel simian immunodeficiency virus (SIVmonNG1) genome sequence from a mona monkey (Cercopithecus mona)
Characterization of a novel simian immunodeficiency virus (SIVmonNG1) genome sequence from a mona monkey (Cercopithecus mona)
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DOI：
10.1128/jvi.77.12.6879-6888.2003
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发表时间：
2003-06-01
影响因子：
5.4
通讯作者：
Clewley, JP
中科院分区：
文献类型：
--
作者：
Barlow, KL;Ajao, AO;Clewley, JP
从出生于尼日利亚的野生猴(CerCopithecus Mona)血清中提取的RNA中，发现了一种新的猴免疫缺陷病毒(SIV)序列。该序列是通过使用新的通用(简并)聚合酶链式反应(PCR)引物获得的，从三分之二进入Gag基因，到SIVmonNG1RNA基因组的3‘聚(A)尾。对开放阅读框的分析表明，SIVmonNG1基因组编码VPU蛋白，此外还有Gag、Pol、Vif、Vpr、Tat、Rev、Env和Nef蛋白。以前，只有感染人类(人类免疫缺陷病毒1型[HIV-1])和黑猩猩(SIVcpz)的慢病毒才有VPU基因；最近，在黑尾猴的SIVgsn中也发现了VPU基因。总体而言，SIVmonNG1与SIVgsn最为相似：env基因序列组中含有HIV-1/SIVcpz env序列，而pol1基因序列与中华绒毛猴SIVsyk的Pol1序列聚在一起。经BootScan和相似性分析，POL的前半部分与SIVsyk相似，后半部分与Colobus guereza的SIVol相近。SIVmonNG1和SIVgsn的复杂镶嵌基因组之间的相似性符合共同或共同的谱系。这些数据进一步突出了来自不同灵长类物种的SIV之间错综复杂的关系，并将有助于解开这些联系。
A novel simian immunodeficiency virus (SIV) sequence has been recovered from RNA extracted from the serum of a mona monkey (Cercopithecus mona) wild born in Nigeria. The sequence was obtained by using novel generic (degenerate) PCR primers and spans from two-thirds into the gag gene to the 3' poly(A) tail of the SIVmonNG1 RNA genome. Analysis of the open reading frames revealed that the SIVmonNG1 genome codes for a Vpu protein, in addition to Gag, Pol, Vif, Vpr, Tat, Rev, Env, and Nef proteins. Previously, only lentiviruses infecting humans (human immunodeficiency virus type 1 [HIV-1]) and chimpanzees (SIVcpz) were known to have a vpu gene; more recently, this has also been found in SIVgsn from Cercopithecus nictitans. Overall, SIVmonNG1 most closely resembles SIVgsn: the env gene sequence groups with HIV-1/SIVcpz env sequences, whereas the pol gene sequence clusters closely with the pol sequence of SIVsyk from Cercopithecus albogaris. By bootscanning and similarity plotting, the first half of pol resembles SIVsyk, whereas the latter part is closer to SIVcol from Colobus guereza. The similarities between the complex mosaic genomes of SIVmonNG1 and SIVgsn are consistent with a shared or common lineage. These data further highlight the intricate nature of the relationships between the SIVs from different primate species and will be helpful for unraveling these associations.