Supplementation with rac-GR24 Facilitates the Accumulation of Biomass and Astaxanthin in Two Successive Stages of Haematococcus pluvialis Cultivation
Supplementation with rac-GR24 Facilitates the Accumulation of Biomass and Astaxanthin in Two Successive Stages of Haematococcus pluvialis Cultivation
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补充 rac-GR24 促进雨生红球藻培养的两个连续阶段中生物量和虾青素的积累
DOI：
10.1021/acs.jafc.2c00479
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发表时间：
2022
影响因子：
6.1
通讯作者：
Hong-Ye Li
中科院分区：
文献类型：
--
作者：
Xiang Wang;Jin-Hua Mou;Zi-Hao Qin;Ting-Bin Hao;Lan Zheng;Joseph Buhagiar;Yu-Hong Liu;Srinivasan Balamurugan;Yuhe He;Carol Sze Ki Lin;Wei-Dong Yang;Hong-Ye Li
单细胞淡水绿色藻类雨生红球藻（Haematococcus pluvialishas）由于其生物合成大量虾青素的能力而吸引了许多研究关注。近年来，人们对天然虾青素的功能进行了大量的研究，但从H.雨养限制了其商业可行性。目前迫切需要通过增加从H.雨育在这项研究中，我们发现，治疗H。pluvialiscultures在开始的macrozooid阶段（第0天）与1 μ Mrac-GR 24，独脚金内酯（一类植物激素）的合成类似物，导致生物量显着增加[最大干细胞重量（DCW）0.53 g/L]在macrozooid阶段和虾青素（从0.63至5.32%的DCW）在血囊阶段。我们阐明，这种增强的生物量积累在大型动物阶段byrac-GR 24是由于其增加的CO2利用效率在光合作用和碳水化合物的生物合成。我们还发现，rac-GR 24刺激过量生产烟酰胺腺嘌呤二核苷酸磷酸（NADPH）和抗氧化酶在H。pluvialiscultures，它减轻了氧化损伤所造成的活性氧物种产生的血囊阶段，由于耗尽氮供应。此外，rac-GR 24对H. pluvialistically改变了脂肪酸生物合成和虾青素酯化途径的活性，这导致rac-GR 24处理的培养物比对照产生更大量的虾青素。总之，我们已经开发出一种可行的和经济的rac-GR 24辅助策略，增加了生物量和虾青素产生的H。雨养，并提供了新的见解的机制作用的rac-GR 24实现这些效果。
The unicellular freshwater green algaHaematococcus pluvialishas attracted much research attention due to its biosynthetic ability for large amounts of astaxanthin, a blood-red ketocarotenoid that is used in cosmetics, nutraceuticals, and pharmaceuticals. Recently, numerous studies have investigated the functions of natural astaxanthin; however, the high cost of the production of astaxanthin fromH. pluvialiscultures restricts its commercial viability. There is an urgent need to fulfill commercial demands by increasing astaxanthin accumulation fromH. pluvialiscultures. In this study, we discovered that treatment ofH. pluvialiscultures at the beginning of the macrozooid stage (day 0) with 1 μMrac-GR24, a synthetic analogue of strigolactones (a class of phytohormones), led to significant increases in biomass [up to a maximum dry cell weight (DCW) of 0.53 g/L] during the macrozooid stage and astaxanthin (from 0.63 to 5.32% of DCW) during the hematocyst stage. We elucidated that this enhancement of biomass accumulation during the macrozooid stage byrac-GR24 is due to its increasing CO2utilization efficiency in photosynthesis and carbohydrate biosynthesis. We also found thatrac-GR24 stimulated the overproduction of nicotinamide adenine dinucleotide phosphate (NADPH) and antioxidant enzymes inH. pluvialiscultures, which alleviated the oxidative damage caused by reactive oxygen species generated during the hematocyst stage due to the exhaustion of nitrogen supplies. Moreover,rac-GR24 treatment ofH. pluvialissynergistically altered the activity of the pathways of fatty acid biosynthesis and astaxanthin esterification, which resulted in larger amounts of astaxanthin being generated byrac-GR24-treated cultures than by controls. In summary, we have developed a feasible and economicrac-GR24-assisted strategy that increases the amounts of biomass and astaxanthin generated byH. pluvialiscultures, and have provided novel insights into the mechanistic roles ofrac-GR24 to achieve these effects.